|Tested species reactivity||Human, Mouse, Rabbit, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full length human caspase 3 protein.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29157 detects Caspase 3 from rat, rabbit, human, and mouse samples.
PA1-29157 has been successfully used in immunoprecipitation, immunohistochemistry (paraffin tissue), and Western blot applications. Possible positive controls are Jurkat cells, and tonsil.
The PA1-29157 immunogen is recombinant full length human caspase 3 protein.
Caspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32 kD) proenzyme. Recent studies have implicated that Caspase 3 is associated with the induction of apoptosis. Activation of Caspase 3 occurs in response to variety of apoptotic inducers including Fas mediated apoptosis. Upon activation, Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176, thereby generating two subunits of 17 kD and 12 kD, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.