Immunofluorescence analysis of Caspase 3 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Caspase 3 [3CSP03 (4.1.18)] Rabbit Polyclonal Antibody (PA516335) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Bovine, Dog, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat|
|Published species reactivity||Dog, Rabbit, Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full length human caspase 3 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 ug/mL|
|Immunoprecipitation (IP)||10 ug/mg protein lysate|
|Western Blot (WB)||2.5-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16335 targets Caspase 3 in immunohistochemistry (paraffin), immunoprecipitation, and western blot applications and shows reactivity with Bovine, Canine, Hamster, Human, mouse, Non-human primate, Ovine, Porcine, Rabbit, and Rat samples.
The PA5-16335 immunogen is recombinant full length human caspase 3 protein.
Caspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32kDa) proenzyme. Upon activation, Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176 thereby generating two subunits of 17kDa and 12kDa, respectively. Recent studies have implicated that Caspase 3 is associated with the induction of apoptosis. Activation of Caspase 3 occurs in response to variety of apoptotic inducers including Fas mediated apoptosis.
IP-MS enrichment of CASP3 (Intensity): CASP3 was enriched 616-fold from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and CASP3 antibody (Part No. PA5-16335). See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cholinergic Surveillance over Hippocampal RNA Metabolism and Alzheimer's-Like Pathology.
PA5-16335 was used in immunohistochemistry to study cholinergic surveillance over Alzheimer's-like pathology and hippocampal RNA metabolism
|Kolisnyk B,Al-Onaizi M,Soreq L,Barbash S,Bekenstein U,Haberman N,Hanin G,Kish MT,Souza da Silva J,Fahnestock M,Ule J,Soreq H,Prado VF,Prado MA||Cerebral cortex (New York, N.Y. : 1991) (null:null)||2016|
Neuroprotective effect of quercetin against oxidative damage and neuronal apoptosis caused by cadmium in hippocampus.
PA5-16335 was used in immunohistochemistry to study the ability of quercetin to protect against cadmium-induced hippocampal oxidative damage and neuronal aopoptosis in a rat model
|Kanter M,Unsal C,Aktas C,Erboga M||Toxicology and industrial health (32:541)||2016|
Role of quercetin in cadmium-induced oxidative stress, neuronal damage, and apoptosis in rats.
PA5-16335 was used in immunohistochemistry to study the beneficial effects of quercetin in a rat model of frontal coretex neuronal injury
|Unsal C,Kanter M,Aktas C,Erboga M||Toxicology and industrial health (31:1106)||2015|
Pretreatment with mineralocorticoid receptor blocker reduces intestinal injury induced by ischemia and reperfusion: involvement of inhibition of inflammatory response, oxidative stress, nuclear factor ¿B, and inducible nitric oxide synthase.
PA5-16335 was used in immunohistochemistry to study the roles of oxidative stress, NFkB and iNOS in the mechanism by which pretreatment with a mineralocorticoid receptor blocker protects against ischemia-reperfusion-induced intestinal injury
|Ozacmak HS,Ozacmak VH,Barut F,Arasl¿ M,Ucan BH||The Journal of surgical research (191:350)||2014|
The effects of orchidectomy on toxicological responses to dietary ochratoxin A in Wistar rats.
PA5-16335 was used in immunohistochemistry to study the effects of castration on ochratoxin A toxicity in rats
|Mor F,Kilic MA,Ozmen O,Yilmaz M,Eker I,Uran K||Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie (66:267)||2014|
Neuroprotective effect of levetiracetam on hypoxic ischemic brain injury in neonatal rats.
PA5-16335 was used in immunohistochemistry to study the ability of levetiracetam to protect against neuronal loss and behavioural deficits in a neonatal rat model of hypoxic-ischemic brain injury
|Komur M,Okuyaz C,Celik Y,Resitoglu B,Polat A,Balci S,Tamer L,Erdogan S,Beydagi H||Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery (30:1001)||2014|
GSPE is superior to NAC in the prevention of contrast-induced nephropathy: might this superiority be related to caspase 1 and calpain 1?
PA5-16335 was used in immunohistochemistry to study the potential role of calpain-1 in the greater protective effect against contrast-induced nephropathy of grape seed proanthocyanidin extract as compared to N-acetyl cysteine
|Ulusoy S,Ozkan G,Mungan S,Orem A,Yulug E,Alkanat M,Yucesan FB||Life sciences (103:101)||2014|
Caspase-3 expression in metastatic lymph nodes of esophageal squamous cell carcinoma is prognostic of survival.
PA5-16335 was used in immunohistochemistry to study the prognostic value of the metastic lymph node expression of caspase-3 in patients with esophageal squamous cell carcinoma
|Wang XS,Luo KJ,Bella AE,Bu SS,Wen J,Zhang SS,Hu Y||World journal of gastroenterology (20:4414)||2014|
Galectin-1 in early acute myocardial infarction.
PA5-16335 was used in immunohistochemistry to study the expression of galectin-1 and HIF-1alpha following myocardial infarction in a murine model
|Al-Salam S,Hashmi S||PloS one (9:null)||2014|
Perspective on rhabdomyolysis-induced acute kidney injury and new treatment options.
PA5-16335 was used in immunohistochemistry to study the protective effects of a grape seed proanthocyanidin extract in a rat model of acute kidney injury induced by rhabdomyolysis
|Ulusoy S,Ozkan G,Alkanat M,Mungan S,Yulu¿ E,Orem A||American journal of nephrology (38:368)||2013|
A single intraperitoneal injection of ketamine does not affect spatial working, reference memory or neurodegeneration in adult mice: An animal study.
PA5-16335 was used in immunohistochemistry to study the effects of a single dose of ketamine on memory and neurodegeneration in an adult murine model
|Ribeiro PO,Rodrigues PC,Valentim AM,Antunes LM||European journal of anaesthesiology (30:618)||2013|
How does colistin-induced nephropathy develop and can it be treated?
PA5-16335 was used in immunohistochemistry to identify the mechanism of colistin-induced nephropathy
|Ozkan G,Ulusoy S,Orem A,Alkanat M,Mungan S,Yulug E,Yucesan FB||Antimicrobial agents and chemotherapy (57:3463)||2013|
Mechanisms of the protective effects of curcumin against indomethacin-induced gastric ulcer in rats.
PA5-16335 was used in immunohistochemistry to study the molecular mechanisms underlying the protective effects of curcumin in a rat gastric ulcer model
|Morsy MA,El-Moselhy MA||Pharmacology (91:267)||2013|
Cardiotoxicity and apoptotic activity in subacute endosulfan toxicity and the protective effect of vitamin C in rabbits: a pathological study.
PA5-16335 was used in immunohistochemistry to study the ability of vitamin C to protect against the cardiopathological changes induced in a rabbit model by subacute doses of endosulfan
|Ozmen O||Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer (32:53)||2013|
The effects of alpha lipoic acid on liver cells damages and apoptosis induced by polyunsaturated fatty acids.
PA5-16335 was used in immunohistochemistry to study the ability of alpha-lipoic acid to protect against the deleterious hepatic effects of a diet high in polyunsaturated fatty acids
|Kaya-Dagistanli F,Tanriverdi G,Altinok A,Ozyazgan S,Ozturk M||Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (53:84)||2013|
Canine tracheal cartilage cryopreservation: freezing injury is not related to caspase-3 expression.
PA5-16335 was used in immunohistochemistry to study the lack of correlation between caspase-3 expression and canine tracheal cartilage cryopreservation damage
|Sotres-Vega A,Santibañez-Salgado JA,Villalba-Caloca J,Gaxiola-Gaxiola M,Ramos-Abraham C,Rosales-Torres AM,Jiménez-García LF||Biopreservation and biobanking (11:45)||2013|
Effects of different ventilation strategies on lung injury in newborn rabbits.
PA5-16335 was used in immunohistochemistry to study the effects of mechanical ventilation and hyperoxia on lung injury in newborns
|Hua S,Zhang X,Zhang S,Xu J,Feng Z||Pediatric pulmonology (47:1103)||2012|
Environmental tobacco smoke exposure during intrauterine period promotes granulosa cell apoptosis: a prospective, randomized study.
PA5-16335 was used in immunohistochemistry to study the induction of granulosa cell apoptosis by intrauterine exposure to tobacco smoke
|Kilic S,Yuksel B,Lortlar N,Sertyel S,Aksu T,Batioglu S||The journal of maternal-fetal and neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians (25:1904)||2012|
Time dependent influence of etonogestrel on the caspase-3 immunoreactivity and apoptotic indexes of rat uterus and ovaries.
PA5-16335 was used in immunohistochemistry to study the time-course of the effects of subcutaneous etonogestrel implants on ovarian and uterine caspase-3 levels and apoptosis in a rat model
|Tasdemir N,Kilic S,Lortlar N,Yuksel B,Goker U,Ozaksit G||Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology (28:463)||2012|
Effect of valproic acid treatment on penile structure in prepubertal rats.
PA5-16335 was used in immunohistochemistry to study the histological effects of valproic acid on the penis in prepubertal rats
|Kutlu O,Cansu A,Karagüzel E,Gürgen SG,Koç O,Gür M,Ozgür GK||Epilepsy research (99:306)||2012|
N-acetylcysteine may prevent severe intestinal damage in necrotizing enterocolitis.
PA5-16335 was used in immunohistochemistry to study the preventative effect of N-acetylcysteine in a rat model of necrotizing enterocolitis
|Tayman C,Tonbul A,Kosus A,Hirfanoglu IM,Uysal S,Haltas H,Tatli MM,Andiran F||Journal of pediatric surgery (47:540)||2012|
Neuroprotective effects of caffeic acid phenethyl ester on experimental traumatic brain injury in rats.
PA5-16335 was used in immunohistochemistry to study the neuroprotective effects of caffeic acid phenethyl ester on experimental traumatic brain injury in rats
|Kerman M,Kanter M,Co¿kun KK,Erboga M,Gurel A||Journal of molecular histology (43:49)||2012|
Temporal changes in PTHrP, Bcl-2, Bax, caspase, TGF-beta, and FGF-2 expression following growth plate irradiation with or without radioprotectant.
PA5-16335 was used in immunohistochemistry to study changes in the expression of apoptosis molecules and growth factors in rats after growth plate irradiation with or without radioprotectant
|Damron TA,Mathur S,Horton JA,Strauss J,Margulies B,Grant W,Farnum CE,Spadaro JA||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (52:157)||2004|
Transplantation of mesenchymal stem cells promotes the functional recovery of the central nervous system following cerebral ischemia by inhibiting myelin-associated inhibitor expression and neural apoptosis.
PA5-16335 was used in immunohistochemistry - paraffin section to investigate the promotion of the functional recovery of the central nervous system following cerebral ischemia through inhibition of myelin-associated inhibitor expression and neural apoptos
|Feng N,Hao G,Yang F,Qu F,Zheng H,Liang S,Jin Y||Experimental and therapeutic medicine (11:1595)||2016|
|Not Applicable||1 µg/ml||
Selective A3 adenosine receptor agonist protects against doxorubicin-induced cardiotoxicity.
PA5-16335 was used in immunohistochemistry - paraffin section to elucidate the function of selective A3 adenosine receptor agonist against doxorubicin-induced cardiotoxicity
|Galal A,El-Bakly WM,Al Haleem EN,El-Demerdash E||Cancer chemotherapy and pharmacology (77:309)||2016|
A novel orally active water-soluble inhibitor of human glutathione transferase exerts a potent and selective antitumor activity against human melanoma xenografts.
PA5-16335 was used in immunohistochemistry - paraffin section to generate and characterize two novel analogues of the anticancer agent 6-((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)thio)hexan-1-ol.
|De Luca A,Rotili D,Carpanese D,Lenoci A,Calderan L,Scimeca M,Mai A,Bonanno E,Rosato A,Geroni C,Quintieri L,Caccuri AM||Oncotarget (6:4126)||2015|
Insights into regulatory molecules of intestinal epithelial cell turnover during experimental infection by Heterophyes heterophyes.
PA5-16335 was used in immunohistochemistry - paraffin section to study the expression of caspase-3 and NF-kB in intestinal epithelia during cell turnover in mice with heterophyiasis
|Ashour DS,Othman AA,Radi DA||Experimental parasitology (143:48)||2014|
Primary blast injury-induced lesions in the retina of adult rats.
PA5-16335 was used in immunohistochemistry - paraffin section to determine the effects of primary blast exposure on the retina
|Zou YY,Kan EM,Lu J,Ng KC,Tan MH,Yao L,Ling EA||Journal of neuroinflammation (10:null)||2013|
Low level primary blast injury in rodent brain.
PA5-16335 was used in immunohistochemistry - paraffin section to perform gene expression and histopathological analyses of low level primary blast injury in rat brain
|Pun PB,Kan EM,Salim A,Li Z,Ng KC,Moochhala SM,Ling EA,Tan MH,Lu J||Frontiers in neurology (2:null)||2011|
Effect of different durations of overdistention on rat bladder function and morphology.
PA5-16335 was used in western blot to study changes in urinary bladder function and morphology following different periods of overdistension and the molecular mechanisms involved
|Jin XD,Cao M,Zhou XL,Yin HP,Chen ZD,Xu N,Jiang H||Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas (45:921)||2012|