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Caspase 9 Polyclonal Antibody
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FIGURE: 1 / 4
Caspase 9 Antibody (BS-0049R) in ICC/IF
HepG2 cells were stained with Caspase 9 Polyclonal Antibody, Unconjugated (bs-0049R) at 1:200 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L), FITC conjugated secondary antibody. DAPI staining of the nucleus in blue.
Product Details
BS-0049R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human Caspase 9 subunit p35, amino acids 11-120.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 that contains a CARD domain. Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Further, Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase 9 promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner, and proteolytically cleaves poly(ADP- ribose) polymerase (PARP). Caspase 9 is expressed in variety of human tissues. An isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. Mutations in Caspase 9 affect brain development. In mice, insufficient Caspase 9 have resulted in abnormal cerebrum development and neuron development. In humans, dysfunctional Caspase 9 expression vary from tissue to tissue. Low levels of Caspase 9 may play a role in cancer development and neurodegenerative diseases such as Alzheimer's Disease (AD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 25 kDa caspase-9 dominant negative protein; APAF-3; apoptosis-related cysteine protease; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; cas9; CASP 9; CASP-9; CASP9; caspase 9, apoptosis-related cysteine peptidase; caspase 9, apoptosis-related cysteine protease; caspase 9b; Caspase-9; caspase-9 dominant negative form; Caspase-9 precursor; caspase-9 variant; caspase-9-carboxyl-terminal divergent; CASPASE-9c; Caspase9; CTD; ICE-LAP6; ICE-like apoptotic protease 6; OTTHUMP00000002324; protein phosphatase 1, regulatory subunit 56; RNCASP9; RP11-265F14.3; unnamed protein product
Gene Aliases: AI115399; APAF-3; APAF3; AW493809; CASP-9; Casp-9-CTD; CASP9; Casp9_v1; Caspase-9; ICE-LAP6; MCH6; PPP1R56
UniProt ID: (Human) P55211, (Mouse) Q8C3Q9
Entrez Gene ID: (Rabbit) 100101592, (Chicken) 426970, (Human) 842, (Dog) 487432, (Mouse) 12371, (Rat) 58918
cysteine-type endopeptidase activity
protein binding
enzyme activator activity
peptidase activity
cysteine-type peptidase activity
hydrolase activity
protein kinase binding
identical protein binding
SH3 domain binding
cysteine-type endopeptidase activity involved in apoptotic signaling pathway
protease
response to hypoxia
kidney development
response to ischemia
proteolysis
apoptotic process
cellular response to DNA damage stimulus
intrinsic apoptotic signaling pathway in response to DNA damage
response to UV
protein processing
platelet formation
response to cobalt ion
response to estradiol
response to lipopolysaccharide
glial cell apoptotic process
cellular response to UV
signal transduction in response to DNA damage
regulation of apoptotic process
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
fibroblast apoptotic process
neuron apoptotic process
protein maturation
cellular response to dexamethasone stimulus
response to indole-3-methanol
leukocyte apoptotic process
response to anesthetic
intrinsic apoptotic signaling pathway
positive regulation of execution phase of apoptosis
epithelial cell apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
response to organic cyclic compound
regulation of response to DNA damage stimulus
aging
response to antibiotic
cellular response to organic cyclic compound
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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