|Tested species reactivity||Bovine, Human, Mouse, Sheep, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding aa 1-134 of human caspase 9|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/mL|
|Immunoprecipitation (IP)||10 µg/ml|
|Western Blot (WB)||2.5-5.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16355 targets Caspase 9 in IHC (P), IP, and WB applications and shows reactivity with Bovine, Human, mouse, Ovine, and Rat samples.
The PA5-16355 immunogen is recombinant protein encoding aa 1-134 of human caspase 9.
Activation of procaspase-9 by Apaf-1 in the cytochrome c/dATP-dependent pathway requires proteolytic cleavage to generate the mature caspase molecule. Deletion of the Apaf-1 WD-40 repeats makes Apaf-1 constitutively active and capable of processing procaspase-9 independent of cytochrome c an dATP. Apaf-1-mediated processing of procaspase-9 occurs at Asp-315 by an intrinsic autocatalytic activity of procaspase-9 itself. Apaf-1 can form oligomers and may facilitate procaspase-9 autoactivation by oligomerizing its precursor molecules. Once activated, caspase-9 can initiate a caspase cascade involving the downstream executioners caspase-3, -6, and -7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.