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Invitrogen
Caspase 9 Polyclonal Antibody, Biotin
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FIGURE: 1 / 5
Caspase 9 Antibody (PA5-16355) in WB
Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), HEL 92.1.7 (Lane 3), K-562 (Lane 4), MDA-MB-231 (Lane 5), PC-3 (Lane 6) and A-431 (Lane 7). The blot was probed with Rabbit Anti-Caspase 9 Polyclonal Antibody, Biotin (Product # PA5-16355, 2 µg/mL) and detected by chemiluminescence. A 46 kDa band corresponding to Caspase 9 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0321B... View More
Product Details
PA5-16355
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encoding aa 1-134 of human caspase 9
Conjugate
Form
Liquid
Concentration
1 mg/mL
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
PA5-16355 targets Caspase 9 in IHC (P), IP, and WB applications and shows reactivity with Bovine, Human, mouse, Ovine, and Rat samples.
The PA5-16355 immunogen is recombinant protein encoding aa 1-134 of human caspase 9.
Target Information
Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 that contains a CARD domain. Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Further, Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase 9 promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner, and proteolytically cleaves poly(ADP- ribose) polymerase (PARP). Caspase 9 is expressed in variety of human tissues. An isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. Mutations in Caspase 9 affect brain development. In mice, insufficient Caspase 9 have resulted in abnormal cerebrum development and neuron development. In humans, dysfunctional Caspase 9 expression vary from tissue to tissue. Low levels of Caspase 9 may play a role in cancer development and neurodegenerative diseases such as Alzheimer's Disease (AD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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