Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunohistochemistry analysis of Caspase 9 showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Caspase 9 Rabbit Polyclonal Antibody (PA516358) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Bovine, Human, Mouse, Sheep, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding aa 1-134 of human caspase 9|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||10µg/mg protein lysate|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16358 targets Caspase 9 in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with Bovine, Human, mouse, Ovine, and Rat samples.
The PA5-16358 immunogen is recombinant protein encoding aa 1-134 of human caspase 9.
Activation of procaspase-9 by Apaf-1 in the cytochrome c/dATP-dependent pathway requires proteolytic cleavage to generate the mature caspase molecule. Deletion of the Apaf-1 WD-40 repeats makes Apaf-1 constitutively active and capable of processing procaspase-9 independent of cytochrome c an dATP. Apaf-1-mediated processing of procaspase-9 occurs at Asp-315 by an intrinsic autocatalytic activity of procaspase-9 itself. Apaf-1 can form oligomers and may facilitate procaspase-9 autoactivation by oligomerizing its precursor molecules. Once activated, caspase-9 can initiate a caspase cascade involving the downstream executioners caspase-3, -6, and -7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Treatment of vitiligo with a chimeric monoclonal antibody to CD20: a pilot study.
PA5-16358 was used in immunohistochemistry to study the potential of a monoclonal anti-CD20 antibody for the treatment of patients with vitiligo
|Ruiz-Argüelles A,García-Carrasco M,Jimenez-Brito G,Sánchez-Sosa S,Pérez-Romano B,Garcés-Eisele J,Camacho-Alarcón C,Reyes-Núñez V,Sandoval-Cruz M,Mendoza-Pinto C,López-Colombo A||Clinical and experimental immunology (174:229)||2013|
Glycyrrhizic acid suppresses the development of precancerous lesions via regulating the hyperproliferation, inflammation, angiogenesis and apoptosis in the colon of Wistar rats.
PA5-16358 was used in immunohistochemistry to the study the mechanisms by which glycyrrhizic acid suppresses the formation of precancerous lesions in the rat colon
|Khan R,Khan AQ,Lateef A,Rehman MU,Tahir M,Ali F,Hamiza OO,Sultana S||PloS one (8:null)||2013|
Expression of Bak and Bak/Mcl-1 ratio can predict photodynamic therapy outcome for oral verrucous hyperplasia and leukoplakia.
PA5-16358 was used in immunohistochemistry to study the utility of the immunohistochemical expression of Bak and Mcl1 in predicting the response of oral verrucous hyperplasia and leukoplakia to photodynamic therapy
|Yu CH,Chen HM,Lin HP,Chiang CP||Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (42:257)||2013|
Effect of valproic acid treatment on penile structure in prepubertal rats.
PA5-16358 was used in immunohistochemistry to study the histological effects of valproic acid on the penis in prepubertal rats
|Kutlu O,Cansu A,Karagüzel E,Gürgen SG,Koç O,Gür M,Ozgür GK||Epilepsy research (99:306)||2012|
N-acetylcysteine may prevent severe intestinal damage in necrotizing enterocolitis.
PA5-16358 was used in immunohistochemistry to study the preventative effect of N-acetylcysteine in a rat model of necrotizing enterocolitis
|Tayman C,Tonbul A,Kosus A,Hirfanoglu IM,Uysal S,Haltas H,Tatli MM,Andiran F||Journal of pediatric surgery (47:540)||2012|
Hsp90-targeted miRNA-liposomal formulation for systemic antitumor effect.
PA5-16358 was used in western blot to study the anti-tumor effects of a system for the GR-mediated delivery of anti-Hsp90 miRNA
|Pore SK,Choudhary A,Rathore B,Ganguly A,Sujitha P,Kumar CG,Agawane SB,Kumar JM,Scaria V,Pillai B,Banerjee R||Biomaterials (34:6804)||2013|
25 kDa caspase-9 dominant negative protein; apoptosis-related cysteine protease; apoptotic protease activating factor 3; apoptotic protease MCH-6; apoptotic protease-activating factor 3; cas9; caspase 9; caspase 9, apoptosis-related cysteine peptidase; caspase-9; caspase-9 dominant negative form; caspase-9-carboxyl-terminal divergent; ICE-like apoptotic protease 6; protein phosphatase 1, regulatory subunit 56
AI115399; APAF-3; APAF3; AW493809; BOS_16018; CASP-9; Casp-9-CTD; CASP9; Casp9_v1; Caspase-9; ICE-LAP6; MCH6; PPP1R56