Immunofluorescence analysis of Caspase 9 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Caspase 9 Rabbit Polyclonal Antibody (PA516358) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Bovine, Human, Mouse, Sheep, Rat|
|Published species reactivity||Rat, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding aa 1-134 of human caspase 9|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Hemagglutination Assay (HA)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||10µg/mg protein lysate|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16358 targets Caspase 9 in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with Bovine, Human, mouse, Ovine, and Rat samples.
The PA5-16358 immunogen is recombinant protein encoding aa 1-134 of human caspase 9.
Activation of procaspase-9 by Apaf-1 in the cytochrome c/dATP-dependent pathway requires proteolytic cleavage to generate the mature caspase molecule. Deletion of the Apaf-1 WD-40 repeats makes Apaf-1 constitutively active and capable of processing procaspase-9 independent of cytochrome c an dATP. Apaf-1-mediated processing of procaspase-9 occurs at Asp-315 by an intrinsic autocatalytic activity of procaspase-9 itself. Apaf-1 can form oligomers and may facilitate procaspase-9 autoactivation by oligomerizing its precursor molecules. Once activated, caspase-9 can initiate a caspase cascade involving the downstream executioners caspase-3, -6, and -7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Geraniol attenuates 2-acetylaminofluorene induced oxidative stress, inflammation and apoptosis in the liver of wistar rats.
PA5-16358 was used in immunohistochemistry - paraffin section to analyze attenuation of 2-acetylaminofluorene induced by oxidative stress, apoptosis and inflammation in the liver of wistar rats by geraniol
|Hasan SK,Sultana S||Toxicology mechanisms and methods (25:559)||2015|
Treatment of vitiligo with a chimeric monoclonal antibody to CD20: a pilot study.
PA5-16358 was used in immunohistochemistry to study the potential of a monoclonal anti-CD20 antibody for the treatment of patients with vitiligo
|Ruiz-Argüelles A,García-Carrasco M,Jimenez-Brito G,Sánchez-Sosa S,Pérez-Romano B,Garcés-Eisele J,Camacho-Alarcón C,Reyes-Núñez V,Sandoval-Cruz M,Mendoza-Pinto C,López-Colombo A||Clinical and experimental immunology (174:229)||2013|
Glycyrrhizic acid suppresses the development of precancerous lesions via regulating the hyperproliferation, inflammation, angiogenesis and apoptosis in the colon of Wistar rats.
PA5-16358 was used in immunohistochemistry to the study the mechanisms by which glycyrrhizic acid suppresses the formation of precancerous lesions in the rat colon
|Khan R,Khan AQ,Lateef A,Rehman MU,Tahir M,Ali F,Hamiza OO,Sultana S||PloS one (8:null)||2013|
Expression of Bak and Bak/Mcl-1 ratio can predict photodynamic therapy outcome for oral verrucous hyperplasia and leukoplakia.
PA5-16358 was used in immunohistochemistry to study the utility of the immunohistochemical expression of Bak and Mcl1 in predicting the response of oral verrucous hyperplasia and leukoplakia to photodynamic therapy
|Yu CH,Chen HM,Lin HP,Chiang CP||Journal of oral pathology and medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (42:257)||2013|
Effect of valproic acid treatment on penile structure in prepubertal rats.
PA5-16358 was used in immunohistochemistry to study the histological effects of valproic acid on the penis in prepubertal rats
|Kutlu O,Cansu A,Karagüzel E,Gürgen SG,Koç O,Gür M,Ozgür GK||Epilepsy research (99:306)||2012|
N-acetylcysteine may prevent severe intestinal damage in necrotizing enterocolitis.
PA5-16358 was used in immunohistochemistry to study the preventative effect of N-acetylcysteine in a rat model of necrotizing enterocolitis
|Tayman C,Tonbul A,Kosus A,Hirfanoglu IM,Uysal S,Haltas H,Tatli MM,Andiran F||Journal of pediatric surgery (47:540)||2012|
Hsp90-targeted miRNA-liposomal formulation for systemic antitumor effect.
PA5-16358 was used in western blot to study the anti-tumor effects of a system for the GR-mediated delivery of anti-Hsp90 miRNA
|Pore SK,Choudhary A,Rathore B,Ganguly A,Sujitha P,Kumar CG,Agawane SB,Kumar JM,Scaria V,Pillai B,Banerjee R||Biomaterials (34:6804)||2013|