Invitrogen

Caspase 9 (active) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

View all (53) Caspase 9 antibodies

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

Antibody Testing Data (3)
Advanced Verification (2)

Caspase 9 (active) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

Caspase 9 (active) Antibody (PA1-26434) in ICC/IF

Immunofluorescence analysis of Caspase 9 was performed using 70% confluent log phase HeLa cells treated with 1µM of Staurosporine for 3 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Caspase 9 Rabbit Polyclonal Antibody (Product # PA1-26434) at 5 mic... View More

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Caspase 9 (active) Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with Staurosporine (1uM for 3hrs) (Lane 2), Jurkat (Lane 3) and Jurkat treated with Staurosporine (1uM for 3hrs) (Lane 4). The blot was probed with Anti-Caspase 9 (active) Polyclonal Antibody (Product # PA1-26434, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 37 kDa band corresponding to cleaved Caspase 9 was observed only in the Staurosporine treated cell lines tested. A non-specific band was also observed at ~58 kDa in all the lanes.

Caspase 9 (active) Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot using Anti-Caspase 9 (active) Polyclonal Antibody (Product # PA1-26434) shows cleaved Caspase 9 protein upon treatment with Staurosporine in HeLa and Jurkat cell lines. A non-specific band was also observed at ~58 kDa in all the lanes. {TM}

Product Details

PA1-26434

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-4 µg/mL

Immunocytochemistry (ICC/IF)

5 µg/mL

Immunoprecipitation (IP)

10-20 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide mapping to the N-terminus adjacent to Asp330 of human caspase-9

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_779546

Product Specific Information

PA1-26434 detects active Caspase 9 from human samples.

Target Information

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 that contains a CARD domain. Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Further, Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase 9 promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner, and proteolytically cleaves poly(ADP- ribose) polymerase (PARP). Caspase 9 is expressed in variety of human tissues. An isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. Mutations in Caspase 9 affect brain development. In mice, insufficient Caspase 9 have resulted in abnormal cerebrum development and neuron development. In humans, dysfunctional Caspase 9 expression vary from tissue to tissue. Low levels of Caspase 9 may play a role in cancer development and neurodegenerative diseases such as Alzheimer's Disease (AD).

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: APAF-3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; cas9; CASP 9; CASP-9; caspase 9, apoptosis-related cysteine peptidase; Caspase-9; Caspase-9 precursor; Caspase-9 subunit p10; Caspase-9 subunit p35; CASPASE-9c; Caspase9; CTD; ICE-LAP6; ICE-like apoptotic protease 6; OTTHUMP00000002324; protein phosphatase 1, regulatory subunit 56; RNCASP9; RP11-265F14.3

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Gene Aliases: CASP9; MCH6