Immunofluorescence analysis of Caveolin 1 was done on 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Caveolin 1 Rabbit Polyclonal Antibody (PA1064) at 1ug/ml in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Dog, Hamster, Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) S G G K Y V D S E G H L Y T V P(17) C of human CAV1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||1-2 µg/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-064 detects caveolin 1 from human, canine, hamster, mouse, and rat tissues and cells.
PA1-064 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects an ~22 kDa protein representing caveolin 1 from rat heart protein extract.
The PA1-064 immunogen is a synthetic peptide corresponding to residues M(1) S G G K Y V D S E G H L Y T V P(17) C of human CAV1. This sequence is completely conserved between human, mouse, canine, bovine, and rat. The PA1-064 immunizing peptide (Cat. # PEP-146) is available for use in neutralization and control experiments.
Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Colocalization of androgen binding protein, oxytocin receptor, caveolin 1 and proliferation marker p21 in benign prostate hyperplasia.
PA1-064 was used in immunohistochemistry to investigate the involvement of androgen and oxytocin receptor in the pathogenesis of benign prostate hyperplasia
|Sendemir E,Herbert Z,Sivukhina E,Zermann DH,Arnold R,Jirikowski GF||Anatomia, histologia, embryologia (37:325)||2008|
Expression of sex hormone-binding globulin, oxytocin receptor, caveolin-1 and p21 in leiomyoma.
PA1-064 was used in immunocytochemistry to investigate the involvement of the oxytocin receptor pathway in leiomyoma
|Sendemir A,Sendemir E,Kosmehl H,Jirikowski GF||Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology (24:105)||2008|
Changing caveolin-1 and oxytocin receptor distribution in the ageing human prostate.
PA1-064 was used in immunocytochemistry to investigate the changes of specific proteins in old human prostate
|Herbert Z,Bötticher G,Aschoff A,Sendemir E,Zermann DH,Arnold R,Mall G,Jirikowski GF||Anatomia, histologia, embryologia (36:361)||2007|
Signaling proteins in raft-like microdomains are essential for Ca2+ wave propagation in glial cells.
PA1-064 was used in western blot to study whether Ca(2+) signaling proteins are organized into lipid-raft microdomains in glial cells
|Weerth SH,Holtzclaw LA,Russell JT||Cell calcium (41:155)||2007|
Isolation of lipid droplets from cells by density gradient centrifugation.
PA1-064 was used in western blot to evaluate a density gradient centrifugation protocol for the isolation of cellular lipid droplets
|Brasaemle DL,Wolins NE||Current protocols in cell biology (Chapter 3:null)||2006|
A high-fat, refined-carbohydrate diet induces endothelial dysfunction and oxidant/antioxidant imbalance and depresses NOS protein expression.
PA1-064 was used in western blot to study effect of diet on endothelial functions
|Roberts CK,Barnard RJ,Sindhu RK,Jurczak M,Ehdaie A,Vaziri ND||Journal of applied physiology (Bethesda, Md. : 1985) (98:203)||2005|
Effects of chronic renal failure on caveolin-1, guanylate cyclase and AKT protein expression.
PA1-064 was used in western blot to investigate the impact of chronic renal failure on expression of Cav-1, sGC and Akt in rats
|Sindhu RK,Ehdaie A,Vaziri ND,Roberts CK||Biochimica et biophysica acta (1690:231)||2004|
Decreased liver fatty acid binding capacity and altered liver lipid distribution in mice lacking the liver fatty acid-binding protein gene.
PA1-064 was used in western blot to investigate the impact of L-FABP mutations on hepatic fatty acid binding capacity, lipid composition, and expression of other lipid-binding proteins.
|Martin GG,Danneberg H,Kumar LS,Atshaves BP,Erol E,Bader M,Schroeder F,Binas B||The Journal of biological chemistry (278:21429)||2003|