|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||1-2 µg/ml|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||1-3 µg/ml|
|Immunocytochemistry (ICC)||See 3 publications below|
|Western Blot (WB)||See 2 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) M T E E H T D L E A R I I K D I H C(19) C of mouse CAV3|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-066 detects Caveolin 3 from rat and mouse tissues. This antibody does not detect caveolin-1 or -2.
PA1-066 has been successfully used in Western blot, IF, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~21 kDa protein representing Caveolin 3 from rat heart homogenate.
The PA1-066 immunogen is a synthetic peptide corresponding to residues M(1) M T E E H T D L E A R I I K D I H C(19) C of mouse CAV3. This sequence is ~85% conserved in human Caveolin 3. PA1-066 immunizing peptide (Cat. # PEP-081) is available for use in neutralization and control experiments.
Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin gene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CAV; CAV3; Caveolin-3; M-caveolin
Gene Aliases: AI385751; Cav-3; CAV3; LGMD1C; LQT9; M-cav; VIP-21; VIP21
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