Immunofluorescent staining of U2OS or HeLa cells with Rb anti-CDC14B (green) (Cat. no. 34-8900). DNA is labeled with DAPI (blue), anti-CDC14B is labeled with FITC (green), and anti-Tubulin is labeled with rhodamine (red). CDC14B appears as bright nuclear dots and midbodies of cells undergoing cytokinesis. Image courtesy of Dr. Catherine Regnard and Dr. Ulrike Grueneberg, Max Planck Institute for Biochemistry, Germany.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the N-terminal region of the human Cdc14B protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CDC14B is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
||The dual-specificity phosphatase CDC14B bundles and stabilizes microtubules.||Cho HP,Liu Y,Gomez M,Dunlap J,Tyers M,Wang Y||Molecular and cellular biology (25:4541)||2005|
|Human||Not Cited||The dual-specificity phosphatase CDC14B bundles and stabilizes microtubules.||Cho HP,Liu Y,Gomez M,Dunlap J,Tyers M,Wang Y||Molecular and cellular biology (25:4541)||2005|