Invitrogen

Cdc20 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Published Figure

2 References

View all (26) CDC20 antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

Cdc20 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 9

Cdc20 Antibody (PA5-63103) in IHC

Immunohistochemical staining of Cdc20 in human testis and skeletal muscle tissues using Cdc20 Polyclonal Antibody (Product # PA5-63103). Corresponding CDC20 RNA-seq data are presented for the same tissues.

Product Image Gallery

Cdc20 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of Cdc20 in human cell line U-2 OS using Cdc20 Polyclonal Antibody (Product # PA5-63103) shows localization to nucleoplasm & cytosol.

Cdc20 Antibody in Western Blot (WB) — Figure 7 ZNF367 activates the cell cycle signaling pathway to promote glioma progression. ( A and B ) a KEGG analysis based on the genes showing a strong relevance (correlation coefficient R > 0.05) to ZNF367 in the TCGA-GBM database; ( C ) correlations between GABPB1-AS1 and the cell cycle-related factors PCNA, CDC20, CDC7 and CCNA1 predicted in the TCGA-GBM database; ( D and E ) protein levels of PCNA, CDC20, CDC7 and CCNA1 in U251 and SHG44 cells examined by Western blot analysis. Data were collected from three independent experiments and expressed as the mean +- SD. Data were analyzed by two-way ANOVA. ** p < 0.01 vs NC group, ## p < 0.01 vs shRNA + Inhibitor NC group. Abbreviations: NC, negative control; shRNA, short hairpin RNA of GABPB1-AS1; PCNA, proliferating cell nuclear antigen; CCNA1, cyclin A1.

Cdc20 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of Cdc20 demonstrates antibody specificity. Immunohistochemical analysis of Cdc20 using anti-Cdc20 Polyclonal Antibody (Product # PA5-63103), shows significant staining of Cdc20 in human testis and shows minimal or weak staining in human prostate tissues. The relative expression levels of Cdc20 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-63103

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human Cdc20

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2639559

Product Specific Information

Immunogen sequence: TPTKKEHQKA WALNLNGFDV EEAKILRLSG KPQNAPEGYQ NRLKVLYSQK ATPGSSRKTC RYIPSLPDRI LDAPEIRNDY

Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 98%.

Target Information

Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MYBL2 in synergy with CDC20 promotes the proliferation and inhibits apoptosis of gastric cancer cells.

Advances in clinical and experimental medicine : official organ Wroclaw Medical University

Deng Q,Wu L,Li Y,Zou L

PA5-63103 was used in Western Blot to examine the role of myeloblastosis viral oncogene homolog-like 2 (MYBL2) in gastric cancer progression and investigate the underlying mechanisms.

Wed Sep 01 00:00:00 EDT 2021

Long Non-Coding RNA GABPB1-AS1 Augments Malignancy of Glioma Cells by Sequestering MicroRNA-330 and Reinforcing the ZNF367/Cell Cycle Signaling Pathway.

Neuropsychiatric disease and treatment

Li X,Wang H

Published figure using Cdc20 polyclonal antibody (Product # PA5-63103) in Western Blot

Sun Apr 24 00:00:00 EDT 2022

Long Non-Coding RNA GABPB1-AS1 Augments Malignancy of Glioma Cells by Sequestering MicroRNA-330 and Reinforcing the ZNF367/Cell Cycle Signaling Pathway.

Neuropsychiatric disease and treatment

Li X,Wang H

Published figure using Cdc20 polyclonal antibody (Product # PA5-63103) in Western Blot

Sun Apr 24 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: CDC20 cell division cycle 20 homolog; cell division cycle 20 homolog; Cell division cycle protein 20 homolog; p55CDC

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Gene Aliases: bA276H19.3; CDC20; CDC20A; p55CDC

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UniProt ID: (Human) Q12834

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Entrez Gene ID: (Human) 991

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Molecular Function: ubiquitin-protein ligase

Function(s)

cell cycle sister chromatid cohesion mitotic nuclear division positive regulation of cell proliferation anaphase-promoting complex-dependent catabolic process positive regulation of synaptic plasticity regulation of meiotic nuclear division protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process regulation of dendrite development cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of synapse maturation positive regulation of ubiquitin protein ligase activity

Process(es)

protein binding protein C-terminus binding anaphase-promoting complex binding enzyme binding histone deacetylase binding ubiquitin-protein transferase activator activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cdc20 Polyclonal Antibody

Cdc20 Antibody (PA5-63103) in IHC

Product Details

PA5-63103

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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