Search
Invitrogen
Cdc25B Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 5
Cdc25B Antibody (PA5-17759) in ICC/IF
Immunofluorescence analysis of CDC25B was performed using 70% confluent log phase HeLa and serum starved HeLa cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with CDC25B Polyclonal Antibody (Product # PA5-17759) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor P... View More
Product Details
PA5-17759
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues at the carboxy terminus of human cdc25B
Conjugate
Form
Liquid
Concentration
278 µg/mL
Amount
27.8 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CDC25 homolog B; Dual specificity phosphatase Cdc25B; EC 3.1.3.48; M-phase inducer phosphatase 2; MPIP2; p63; putative; unnamed protein product
Gene Aliases: AI604853; CDC25B; CDC25HU2; Cdc25m2; MPIP2
UniProt ID: (Human) P30305, (Mouse) P30306, (Rat) P48966
Entrez Gene ID: (Human) 994, (Mouse) 12531, (Rat) 171103
G2/M transition of mitotic cell cycle
mitotic cell cycle
oocyte maturation
protein phosphorylation
female meiosis I
positive regulation of cell proliferation
positive regulation of G2/M transition of mitotic cell cycle
positive regulation of cytokinesis
positive regulation of mitotic cell cycle
cell division
positive regulation of G2/MI transition of meiotic cell cycle
positive regulation of cell cycle G2/M phase transition
mitotic M phase
protein dephosphorylation
cell cycle
mitotic nuclear division
positive regulation of protein kinase activity
peptidyl-tyrosine dephosphorylation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support