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Invitrogen
Cdc42 Polyclonal Antibody
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FIGURE: 1 / 5
Cdc42 Antibody (PA5-20764) in ICC/IF
Immunofluorescence analysis of cdc42 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cdc42 Polyclonal Antibody (Product # PA5-20764) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 488 (Product # A-11039) at a dilution of 1:2000 for... View More
Product Details
PA5-20764
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
A 17 amino acid peptide near the amino terminus of human CDC42.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is human brain tissue lysate.
PA5-20764 can be used with blocking peptide PEP-0878.
Antibodies should not be exposed to prolonged high temperatures.
Target Information
Rac1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for the Rac1 gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell division control protein 42 homolog; cell division cycle 42 (GTP binding protein); cell division cycle 42 homolog; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; GTP-binding protein; RP1-224A6.5; small GTP binding protein CDC42
Gene Aliases: AI747189; AU018915; CDC42; CDC42Hs; G25K; TKS
UniProt ID: (Human) P60953, (Rat) Q8CFN2, (Mouse) P60766
Entrez Gene ID: (Human) 998, (Rat) 64465, (Mouse) 12540
GTPase activity
protein binding
GTP binding
protein kinase binding
GTP-dependent protein binding
mitogen-activated protein kinase kinase kinase binding
thioesterase binding
apolipoprotein A-I receptor binding
identical protein binding
Rho GDP-dissociation inhibitor binding
ubiquitin protein ligase activity
nucleotide binding
small GTPase
sprouting angiogenesis
cardiac conduction system development
keratinocyte development
Golgi organization
regulation of mitotic nuclear division
nuclear migration
establishment or maintenance of cell polarity
small GTPase mediated signal transduction
blood coagulation
positive regulation of gene expression
negative regulation of gene expression
protein ubiquitination
substantia nigra development
actin cytoskeleton organization
macrophage differentiation
positive regulation of cell growth
hair follicle morphogenesis
positive regulation of pseudopodium assembly
T cell costimulation
negative regulation of protein complex assembly
keratinization
regulation of protein stability
positive regulation of cytokinesis
positive regulation of peptidyl-serine phosphorylation
adherens junction organization
cellular protein localization
establishment or maintenance of apical/basal cell polarity
multicellular organism growth
dendritic cell migration
Fc-gamma receptor signaling pathway involved in phagocytosis
negative regulation of epidermal growth factor receptor signaling pathway
regulation of protein catabolic process
regulation of protein heterodimerization activity
positive regulation of neuron apoptotic process
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of DNA replication
regulation of protein kinase activity
positive regulation of JNK cascade
filopodium assembly
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
neuron fate determination
actin filament bundle assembly
regulation of small GTPase mediated signal transduction
positive regulation of muscle cell differentiation
regulation of filopodium assembly
establishment of Golgi localization
positive regulation of synapse structural plasticity
regulation of attachment of spindle microtubules to kinetochore
heart contraction
canonical Wnt signaling pathway
Wnt signaling pathway, planar cell polarity pathway
positive regulation of epithelial cell proliferation involved in lung morphogenesis
submandibular salivary gland formation
epithelial-mesenchymal cell signaling
hair follicle placode formation
positive regulation of hair follicle cell proliferation
organelle transport along microtubule
actin filament branching
epithelial cell-cell adhesion
positive regulation of intracellular protein transport
positive regulation of substrate adhesion-dependent cell spreading
positive regulation of protein phosphorylation
endocytosis
actin filament organization
metabolic process
response to glucose
single organismal cell-cell adhesion
regulation of exocytosis
establishment of cell polarity
positive regulation of catalytic activity
positive regulation of MAPK cascade
epidermis morphogenesis
regulation of protein metabolic process
nucleus localization
Rho protein signal transduction
nervous system development
endosomal transport
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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