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Invitrogen
Cdc6 Polyclonal Antibody
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FIGURE: 1 / 3
Cdc6 Antibody (PA5-95150) in WB
Western Blot was performed using Anti-Cdc6 Polyclonal Antibody (Product # PA5-95150) and a 62 kDa band corresponding to CDC6 was observed in tissue extracts and showed differential expression upon treatments in cell lines. Whole cell extracts (40 µg lysate) from asynchronous HeLa (Lane 1), serum starved HeLa (0.1% FBS for 72 hr) (Lane 2), serum starved (0.1% FBS for 72 hr) and serum released HeLa (10% FBS for 20 hr) (Lane 3), asynchronous T98G (Lane 4), serum starved and released T98G (Lane 5), serum starved T98G (Lane 6), untreated HCT-11... View More
Product Details
PA5-95150
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cdc6 (484-503aa KLYEAYSKVCRKQQVAAVDQ).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cdc18-related protein; CDC6 cell division cycle 6 homolog; CDC6-related protein; Cell division control protein 6 homolog; cell division cycle 6 homolog; HsCdc18; HsCDC6; p62(cdc6); unnamed protein product
Gene Aliases: CDC18L; CDC6; HsCDC18; HsCDC6; MGORS5
UniProt ID: (Human) Q99741
Entrez Gene ID: (Human) 990
DNA replication checkpoint
regulation of cyclin-dependent protein serine/threonine kinase activity
DNA replication
DNA replication initiation
traversing start control point of mitotic cell cycle
negative regulation of DNA replication
negative regulation of cell proliferation
regulation of mitotic metaphase/anaphase transition
positive regulation of cytokinesis
mitotic DNA replication checkpoint
positive regulation of fibroblast proliferation
cell division
positive regulation of chromosome segregation
cellular response to vasopressin
cellular response to angiotensin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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