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Invitrogen
Cdc73 Polyclonal Antibody
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FIGURE: 1 / 1
Cdc73 Antibody (PA5-95272) in WB
Western blot analysis of CDC73 using Cdc73 Polyclonal Antibody (Product # PA5-95272). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel)/120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human Hela whole cell lysates. Lane 2: human MCF-7 whole cell lysates. Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat... View More
Product Details
PA5-95272
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human HRPT2 recombinant protein (Position: Q119-R520).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Human HRPT2 shares 100% and 99.8% amino acid (aa) sequence identity with mouse and rat HRPT2, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human MCF-7 whole cell, human Jurkat whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Cdc73 is a tumor suppressor probably involved in transcriptional and post-transcriptional control pathways. Cdc73 is part of the PAF1 complex, a five-subunit protein complex composed of Paf1, Cdc73, Leo1, Rtf1 and Ctr9. PAF1 was first purified from yeast in association with RNA polymerase II and is believed to function in transcription elongation. The yeast PAF (yPAF) complex interacts with RNA polymerase II and coordinates the setting of histone marks associated with active transcription. In addition to coordinating events during transcription (initiation, promoter clearance, and elongation), hPAF also coordinates events in RNA quality control. Cdc73 may also be involved in cell cycle progression through the regulation of cyclin D1/PRAD1 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell division cycle 73 Paf1/RNA polymerase II complex component homolog (S. cerevisiae) isoform CDC73_1; cell division cycle 73 Paf1/RNA polymerase II complex component homolog (S. cerevisiae) isoform CDC73_2; cell division cycle 73 Paf1/RNA polymerase II complex component-like protein; cell division cycle 73, Paf1/RNA polymerase II complex component, homolog; Cell division cycle protein 73 homolog; Familial isolated hyperparathyroidism; Hyperparathyroidism 2 protein; Paf1/RNA polymerase II complex component; Parafibromin; unnamed protein product
Gene Aliases: C1orf28; CDC73; FIHP; HPTJT; HRPT1; HRPT2; HYX
UniProt ID: (Human) Q6P1J9
Entrez Gene ID: (Human) 79577
negative regulation of transcription from RNA polymerase II promoter
regulation of cell growth
endodermal cell fate commitment
regulation of transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
negative regulation of cell proliferation
Wnt signaling pathway
stem cell population maintenance
positive regulation of Wnt signaling pathway
mRNA 3'-end processing
positive regulation of mRNA 3'-end processing
protein destabilization
positive regulation of transcription elongation from RNA polymerase II promoter
negative regulation of apoptotic process
negative regulation of myeloid cell differentiation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of fibroblast proliferation
negative regulation of epithelial cell proliferation
cellular response to lipopolysaccharide
positive regulation of cell cycle G1/S phase transition
negative regulation of G1/S transition of mitotic cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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