Invitrogen

Claudin 1 Polyclonal Antibody (MH25)

Product Image Gallery

Claudin 1 Antibody in Western Blot (WB) — Western blot analysis using Rabbit anti-Claudin-1 Polyclonal Antibody in: Lane 1: Rat liver. Lane 2: Rat kidney. Lane 3: MDCK cells. Lane 4: Caco-2 cells. Lane 5: Mouse hepatocytes (Product # 71-7800).

Claudin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Claudin-1 showing staining in the membrane and weak cytoplasm staining of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Claudin-1 Rabbit Polyclonal Antibody (Product # 71-7800) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Claudin 1 Antibody in Immunofluorescence (IF) — Immunofluorescent staining of rat small intestine using Rb anti-Claudin-1 (Product # 71-7800).

Claudin 1 Antibody — Figure 10 Claudin-2 expression is limited to the base colon of crypt in NKCC1 WT/DFX and NKCC1 DFX/DFX mice. Representative IF images of NKCC1 WT/WT , NKCC1 WT/DFX , and NKCC1 DFX/DFX mouse colon sections stained with ( A-C ) claudin-2 and ( D-F ) claudin-1 antibodies (red). Sections also show DAPI (blue) or nuclei staining. ( D-F ) In contrast, claudin-1 expression at the lateral membrane was similar in all 3 genotypes. Scale bars : 20 mum. Number of claudin-2-positive cells per crypt were counted on 5 fields, 11 crypts per genotype. There was a significant decrease in the number of cells expressing claudin-2 in the mutant mice. P = .043 for NKCC1-DFX and P = .019 for KO. There was no difference between NKCC1-DFX and NKCC1 KO mice ( P = .93). ( G ) Data are shown as whisker boxes and statistical significance was calculated by 1-way analysis of variance followed by Tukey posttests. CLDN2, claudin-2.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 44 publications 44 publications

Immunohistochemistry (IHC)

View 14 publications 14 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC)

View 17 publications 17 publications

Immunofluorescence (IF)

15-20 µg/mL

View 26 publications 26 publications

ELISA (ELISA)

0.1-1.0 µg/mL

Immunoprecipitation (IP)

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 6 publications 6 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Artificial Control, Avian, Bovine, Dog, Fish, Human, Mouse, Rabbit, Rat, Tag, Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Clone

MH25

Immunogen

A synthetic peptide derived from the C-terminus of the human/mouse Claudin-1 protein.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533997

Product Specific Information

This antibody reacts with the ~ 22 kDa Claudin-1 protein. Reactivity was confirmed by western blotting and immunofluorescence. Positive controls include MDCK cells (dog), Caco-2cells (human), mouse liver, rat liver, and rat kidney.

NOTE: This antibody strongly cross reacts with the Claudin-3 protein. It has been observed in western blotting inhibition studies using Caco-2 cell lysates, that this antibody is inhibited by addition of Claudin-3 peptide. For a Claudin-1 specific antibody that does not cross-react with Claudin-3, please order Cat. No. 519000 (Rb x Claudin-1) instead.

Target Information

Claudin-1 is a member of the transmembrane protein family claudins located in cell-cell tight junctions and it acts as a co-receptor for HCV entry into hepatic cells. Claudins are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Claudin-1 is expressed in most tissues such as bladder, fallopian tube, liver, pancreas, prostate, and skin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.