Immunohistochemistry analysis of Claudin-10 showing staining in the membrane of paraffin-embedded rat kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Claudin-10 polyclonal antibody (388400) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse, Rhesus monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from the C-terminus of the human/mouse Claudin-10 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Immunofluorescence (IF)||See 2 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 1 publications below|
Claudin proteins are a family of proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Claudin-6 enhances cell invasiveness through claudin-1 in AGS human adenocarcinoma gastric cancer cells.
38-8400 was used in flow cytometry to ascertain how claudin-6 is associated with MMP-2 activation and cell invasiveness
|Torres-Martínez AC,Gallardo-Vera JF,Lara-Holguin AN,Montaño LF,Rendón-Huerta EP||Experimental cell research (350:226)||2017|
Immunohistological characterization of intercellular junction proteins in rhesus macaque intestine.
38-8400 was used in immunohistochemistry to examine the localization of epithelial junctions in relation to paracellular permeability and intestinal adhesion
|Gumber S,Nusrat A,Villinger F||Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie (66:437)||2014|
Claudins 10 and 18 are predominantly expressed in lung adenocarcinomas and in tumors of nonsmokers.
38-8400 was used in immunohistochemistry to measure claudins 8 and 0 expression in primary lung carcinomas.
|Merikallio H,Pääkkö P,Harju T,Soini Y||International journal of clinical and experimental pathology (4:667)||2011|
|Mouse||Not Cited||Deletion of claudin-10 (Cldn10) in the thick ascending limb impairs paracellular sodium permeability and leads to hypermagnesemia and nephrocalcinosis.||Breiderhoff T,Himmerkus N,Stuiver M,Mutig K,Will C,Meij IC,Bachmann S,Bleich M,Willnow TE,Müller D||Proceedings of the National Academy of Sciences of the United States of America (109:14241)||2012|
|Rat||2.5 µg/ml||Differences in claudin synthesis in primary cultures of acinar cells from rat salivary gland are correlated with the specific three-dimensional organization of the cells.||Qi B,Fujita-Yoshigaki J,Michikawa H,Satoh K,Katsumata O,Sugiya H||Cell and tissue research (329:59)||2007|
Molecular staging of epithelial maturation using secretory cell-specific genes as markers.
38-8400 was used in immunohistochemistry - frozen section to identify Clara cell-specific genes using chemical and transgenic approaches to ablate Clara cells.
|Zemke AC,Snyder JC,Brockway BL,Drake JA,Reynolds SD,Kaminski N,Stripp BR||American journal of respiratory cell and molecular biology (40:340)||2009|
|Human||Not Cited||Claudin-1, -2, -3, -4, -7, -8, and -10 protein expression in biliary tract cancers.||Németh Z,Szász AM,Tátrai P,Németh J,Gyorffy H,Somorácz A,Szíjártó A,Kupcsulik P,Kiss A,Schaff Z||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (57:113)||2009|