Immunofluorescence analysis of Claudin-12 was done on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Claudin-12 rabbit polyclonal Antibody (388200) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing junctional localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the N-terminal region of mouse and human Claudin-12|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The claudin superfamily consists of many structurally related proteins in humans. These proteins, which include claudin 1 through 18, are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Claudins, which consist of four trans-membrane domains and two extracellular loops, make up tight junction strands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Expression of Tight Junction Proteins and Cadherin 17 in the Small Intestine of Young Goats Offered a Reduced N and/or Ca Diet.
38-8200 was used in western blot to characterize a reduced N and/or Ca diet by expression of tight junction proteins and cadherin 17 in the small intestine of young goats
|Elfers K,Marr I,Wilkens MR,Breves G,Langeheine M,Brehm R,Muscher-Banse AS||PloS one (11:null)||2016|
|Not Applicable||Not Cited||
Vitamin D receptor knockout mice exhibit elongated intestinal microvilli and increased ezrin expression.
38-8200 was used in western blot to characterize the exhibition of elongated intestinal microvilli and increased ezrin expression due to vitamin D receptor knockout mice
|Kühne H,Hause G,Grundmann SM,Schutkowski A,Brandsch C,Stangl GI||Nutrition research (New York, N.Y.) (36:184)||2016|
|Not Applicable||Not Cited||
Distinct molecular composition of blood and lymphatic vascular endothelial cell junctions establishes specific functional barriers within the peripheral lymph node.
38-8200 was used in immunohistochemistry to examine the architecture of cellular junctions present in blood and lymphatic vessel endothelium in peripheral lymph nodes
|Pfeiffer F,Kumar V,Butz S,Vestweber D,Imhof BA,Stein JV,Engelhardt B||European journal of immunology (38:2142)||2008|