|Tested species reactivity||Dog, Human|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal region of human claudin-8|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Claudin-8 belongs to the claudin family of integral membrane proteins. Claudins are responsible for the formation of tight junction strands, and are connected with the actin cytoskeleton mediated by ZO-1. Claudin-8 is a tight junction protein expressed in several distal nephron segments, suggesting that it may have a role as a paracellular cation barrier protein. Claudin-8 is expressed in the inner ear, and the umbrella cell tight junctions in the bladder epithelium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Human||1:350||Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies.||Collins JE,Kirk A,Campbell SK,Mason J,Wilson SJ||Methods in molecular biology (Clifton, N.J.) (762:371)||2011|
|Human||Not Cited||beta-catenin expression and claudin expression pattern as prognostic factors of prostatic cancer progression.||Szász AM,Nyirády P,Majoros A,Szendrõi A,Szûcs M,Székely E,Tõkés AM,Romics I,Kulka J||BJU international (105:716)||2010|
|Human||Not Cited||Claudin-1, -2, -3, -4, -7, -8, and -10 protein expression in biliary tract cancers.||Németh Z,Szász AM,Tátrai P,Németh J,Gyorffy H,Somorácz A,Szíjártó A,Kupcsulik P,Kiss A,Schaff Z||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (57:113)||2009|
|Rat||2.5 µg/ml||Differences in claudin synthesis in primary cultures of acinar cells from rat salivary gland are correlated with the specific three-dimensional organization of the cells.||Qi B,Fujita-Yoshigaki J,Michikawa H,Satoh K,Katsumata O,Sugiya H||Cell and tissue research (329:59)||2007|