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Invitrogen
Cofilin Polyclonal Antibody
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FIGURE: 1 / 5
Cofilin Antibody (PA5-17372) in ICC/IF
Immunofluorescence analysis of Cofilin was performed using 70 confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cofilin Polyclonal Antibody (Product # PA5-17372) at 1:1000 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjug... View More
Product Details
PA5-17372
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues surrounding Asp59 of human cofilin
Conjugate
Form
Liquid
Concentration
20 µg/mL
Amount
2 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 18 kDa phosphoprotein; CFL-1; COF1; cofilin 1 (non-muscle); Cofilin, non-muscle isoform; Cofilin-1; Cofilin1; epididymis secretory protein Li 15; p18; unnamed protein product
Gene Aliases: AA959946; CFL; CFL1; Cof; cofilin; HEL-S-15; n-cofilin
UniProt ID: (Bovine) Q5E9F7, (Human) P23528, (Rat) P45592, (Mouse) P18760
Entrez Gene ID: (Bovine) 534553, (Human) 1072, (Rat) 29271, (Mouse) 12631
cytoskeleton organization
regulation of cell morphogenesis
mitotic cytokinesis
neural crest cell migration
neural fold formation
protein import into nucleus
actin filament organization
negative regulation of cell adhesion
Rho protein signal transduction
actin polymerization or depolymerization
response to virus
positive regulation of lamellipodium assembly
negative regulation of lamellipodium assembly
response to activity
hippocampus development
establishment of cell polarity
cell projection organization
actin cytoskeleton organization
actin filament depolymerization
actin filament fragmentation
positive regulation of cell growth
negative regulation of actin filament depolymerization
positive regulation of actin filament depolymerization
positive regulation of synaptic plasticity
negative regulation of actin filament bundle assembly
positive regulation of embryonic development
negative regulation of apoptotic process
response to amino acid
positive regulation by host of viral process
negative regulation of cell size
positive regulation of proteolysis
cell motility
modulation of synaptic transmission
actin filament severing
establishment of spindle localization
negative regulation of unidimensional cell growth
positive regulation of focal adhesion assembly
positive regulation of dendritic spine development
regulation of dendritic spine morphogenesis
cellular response to hydrogen peroxide
cellular response to interleukin-1
cellular response to interleukin-6
cellular response to tumor necrosis factor
cellular response to ether
cellular response to epidermal growth factor stimulus
modification of postsynaptic actin cytoskeleton
negative regulation of dendritic spine maintenance
positive regulation of protein localization to cell leading edge
negative regulation of postsynaptic density organization
cellular response to insulin-like growth factor stimulus
negative regulation of cell motility
positive regulation of cell motility
positive regulation of establishment of cell polarity regulating cell shape
positive regulation of barbed-end actin filament capping
protein phosphorylation
movement of cell or subcellular component
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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