Invitrogen

Collagen I Polyclonal Antibody

Product Image Gallery

Collagen I Antibody — Figure 9 Omegaven(r) inhibits myofibroblast markers induced by TGFbeta1. Human liver epithelial cell line THLE-3 was incubated in presence or absence of lipid emulsions Omegaven(r) 10%, Lipofundin MCT/LCT(r) 20%, ClinOleic(r) 20% or SMOFlipid(r) 20% at different dilutions, for 30 min followed by TGFbeta1 5 ng/mL stimulation for additional 72 hours. (A) Visible morphology and immunofluorescence for alpha smooth muscle actin (alphaSMA) and collagen type I (col type I) distribution and expression. B) Expression of mRNA of alphaSMA and col type I . Scale bar: 10 um. Results are expressed as means +- SEM of six independent experiments. * p <0.05 related to the control group. # p <0.05 related to the stimulus.

Collagen I Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Collagen I in formalin-fixed, paraffin-embedded liver tissue using a Collagen I polyclonal antibody (Product # PA1-26204) at a dilution of 1:250 for 4°C for 24 hrs and detected using a Peroxidase biotin-streptavidin rabbit secondary antibody at a dilution of 1:10,000 for 45 min at room temperature. A: Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Antigen retrieval was not required. Collagen type I is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.

Product Details

Tested Applications

Dilution

ELISA (ELISA)

1:5000-1:50000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunoprecipitation (IP)

1:100

Western Blot (WB)

1:5000-1:10,000

Published Applications

Immunohistochemistry (Paraffin) (IHC (P))

See 1 publication below

Immunofluorescence (IF)

See 1 publication below

Immunocytochemistry (ICC)

See 1 publication below

Western Blot (WB)

See 4 publications below

Dot blot (DB)

See 1 publication below

Immunohistochemistry (IHC)

See 2 publications below

Product Specifications

Species Reactivity

Human, Rat

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Collagen type I extracted and purified from human and bovine placenta by limited pepsin digestion and selective salt precipitation.

Conjugate

Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.125M sodium borate, pH 8.0, with 5mM EDTA, 0.075M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2260734

Product Specific Information

PA1-26204 detects Collagen I from human and rat samples. This antibody exhibits minimal cross reactivity with Collagens 2-6.

PA1-26204 has been successfully used in immunoprecipitation, ELISA, Immunohistochemistry (fozen, paraffin) and Western blot procedures. Perform Western blot on non-denatured, non-dissociating protein samples.

The PA1-26204 immunogen is Collagen type I extracted and purified from human and bovine placenta by limited pepsin digestion and selective salt precipitation.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Alpha-1 type I collagen; alpha1(I) procollagen; Collagen 1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone, alpha-1 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; Type I Collagen; type I proalpha 1; type I procollagen alpha 1 chain; type I-alpha 1 collagen

Gene Aliases: COL1A1; COLIA1; EDSC; OI1; OI2; OI3; OI4

UniProt ID: (Human) O76045, (Rat) P02454

Entrez Gene ID: (Human) 1277, (Rat) 29393

Function(s)