Invitrogen

Collagen I Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

9 Published Figures

23 References

View all (26) Collagen I antibodies

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Datasheet

Protocols

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Collagen I Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of Collagen I was performed in formalin fixed, paraffin embedded normal kidney using Collagen I Polyclonal Antibody (Product # PA1-26204) at a dilution of 1:100 for 4 hours at room temperature. A peroxidase goat anti-rabbit was used as a secondary antibody at 1:10,000 for 45 min at RT. Localization: Distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain. Antigen retrieval: No antigen retrieval was performed.

Collagen I Antibody in Western Blot (WB) — Western blot analysis of Human Collagen Type I was performed by loading 50 ng of Human Collagen Type 1 onto a membrane and blotting with a Collagen I Polyclonal Antibody (Product # PA1-26204) at a dilution of 1:1,000 overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 139 & 130 kDa.

Collagen I Antibody in Immunocytochemistry (ICC/IF) — Figure 2 Collagen I deposition, localization, and quantification in decellularized scaffolds of HDF and JIMT-1/HDF 3D cultures. ( A - P ) Single confocal plane images of collagen I deposition taken in the centre of decellularized 3D cultures incubated in the absence (control) or presence of 5 ng/ml TGF-beta1. The 3D cultures were decellularized, fixed in 3.7% formaldehyde and stained to visualize fibronectin (green) at 3, 7, 10, and 14 days of culturing. All images are representative of 3 independent experiments. Scale bar is 50 mum. Quantification of fluorescence intensity using ImageJ in images taken by epifluorescence microscopy in HDF decellularized scaffolds ( Q ) and JIMT-1/HDF decellularized scaffolds ( R ). The columns are represented as mean +- SEM (n = 3).

Collagen I Antibody in Immunohistochemistry (IHC) — FIG. 4. IHC comparison of protein expression between bioprinted tissues and native human skin show comparable morphology. Tight junction proteins ZO-1, Claudin I, and E-Cadherin. Epidermal differentiation proteins Keratin 1, Filaggrin, and Cytokeratin 15. ECM proteins Collagen I and Collagen VII and cell proliferation marker Ki67. Scale bar, 100 mum. ECM, extracellular matrix; IHC, immunohistochemistry.

Collagen I Antibody in Flow Cytometry (Flow) — Figure 7 Alterations in collagen levels of juvenile NDHF cells following a 3-day exposure to microgravity: Immunofluorescence images of 1 g -control cells, RPM-AD and RPM-MCS of collagen-I ( A - C ) and collagen-IV ( D - F ). Transcriptional and translational collagen type I analysis: Quantitative gene expression levels of COL1A1 ( G ), intracellular collagen-I levels ( H ) and flow cytometric analysis of collagen type I-labeled cells ( I ). Quantitative gene expression levels of COL4A5 ( J ), intracellular collagen-I levels ( K ) and flow cytometric analysis of fibronectin-labeled cells ( L ). Flow cytometric analysis of collagen type III-labeled cells ( M ). Quantitative gene expression levels of TIMP1 ( N ), MMP1 ( O ), MMP3 ( P ) and MMP14 ( Q ). Full-length blots of cropped Western blot images are presented in Supplementary Fig. S6 . *p < 0.05 1 g vs. RPM; # p < 0.05 AD vs. MCS. Scale bars: 50 um.

Collagen I Antibody — ADAM9 expression and activity in PDAC. (A) Representative immunohistochemical staining of ADAM9 in PDAC patients showing differential ADAM9 expression. {TM}

Product Details

PA1-26204

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:10,000	View 10 publications 10 publications
Immunohistochemistry (IHC)	-	View 5 publications 5 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	-	View 4 publications 4 publications
Flow Cytometry (Flow)	-	View 1 publication 1 publication
ELISA (ELISA)	1:5,000-1:50,000	-
Immunoprecipitation (IP)	1:100	-
Dot blot (DB)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Bovine, Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Collagen type I extracted and purified from human and bovine placenta by limited pepsin digestion and selective salt precipitation.
Conjugate	Unconjugated
Form	Liquid
Concentration	1.15 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.125M sodium borate, pH 8.0, with 5mM EDTA, 0.075M NaCl
Contains	0.01% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2260734

Product Specific Information

Some class-specific anti-collagens are specific for three-dimensional epitopes, resulting in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Cross-reactivity with other human serum proteins and non-collagen extracellular matrix proteins is neglible.|Western blot: this preparation results in a native conformation of the protein. It is recommend not to add 2-ME or DTT in lysis buffer and not boiling the sample.

Target Information

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p.

Theranostics

Zhao H,Yang H,Geng C,Chen Y,Tang Y,Li Z,Pang J,Shu T,Nie Y,Liu Y,Jia K,Wang J

PA1-26204 was used in Immunohistochemistry to demonstrate that elevated IgE promotes pathological cardiac fibrosis by modulating miR-486a-5p and downstream factors, such as Smad1.

Wed Jul 28 00:00:00 EDT 2021

Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p.

Theranostics

Zhao H,Yang H,Geng C,Chen Y,Tang Y,Li Z,Pang J,Shu T,Nie Y,Liu Y,Jia K,Wang J

PA1-26204 was used in Immunohistochemistry to demonstrate that elevated IgE promotes pathological cardiac fibrosis by modulating miR-486a-5p and downstream factors, such as Smad1.

Wed Jul 28 00:00:00 EDT 2021

Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds.

Scientific reports

Malakpour-Permlid A,Buzzi I,Hegardt C,Johansson F,Oredsson S

PA1-26204 was used in Immunocytochemistry-immunoflourescence to investigate ECM components produced by the cells in the artificial 3D tumour, which is an important step in validating the model.

Tue Mar 23 00:00:00 EDT 2021

Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds.

Scientific reports

Malakpour-Permlid A,Buzzi I,Hegardt C,Johansson F,Oredsson S

PA1-26204 was used in Immunocytochemistry-immunoflourescence to investigate ECM components produced by the cells in the artificial 3D tumour, which is an important step in validating the model.

Tue Mar 23 00:00:00 EDT 2021

Deletion of fibroblast activation protein provides atheroprotection.

Cardiovascular research

Stein S,Weber J,Nusser-Stein S,Pahla J,Zhang HE,Mohammed SA,Oppi S,Gaul DS,Paneni F,Tailleux A,Staels B,von Meyenn F,Ruschitzka F,Gorrell MD,Lüscher TF,Matter CM

PA1-26204 was used in Western Blotting to assess the effects of constitutive Fap deletion on plaque formation in atherosclerosis-prone apolipoprotein E or low-density lipoprotein receptor knockout mice.

Sun Mar 21 00:00:00 EDT 2021

Real-time monitoring of liver fibrosis through embedded sensors in a microphysiological system.

Nano convergence

Farooqi HMU,Kang B,Khalid MAU,Salih ARC,Hyun K,Park SH,Huh D,Choi KH

PA1-26204 was used in Immunocytochemistry-immunoflourescence to investigate if chip embedded electrochemical sensors could be used as a potential substitute for conventional end-point assays for studying fibrosis in microphysiological systems.

Tue Feb 02 00:00:00 EST 2021

Late-life voluntary wheel running reverses age-related aortic stiffness in mice: a translational model for studying mechanisms of exercise-mediated arterial de-stiffening.

GeroScience

Gioscia-Ryan RA,Clayton ZS,Fleenor BS,Eng JS,Johnson LC,Rossman MJ,Zigler MC,Evans TD,Seals DR

PA1-26204 was used in Western Blot to investigate the role of voluntary wheel running as a murine experimental animal exercise suitable for studying the effects of exercise on human arterial stiffness.

Mon Feb 01 00:00:00 EST 2021

Inhibition of Cathepsin K Alleviates Autophagy-Related Inflammation in Periodontitis-Aggravating Arthritis.

Infection and immunity

Yue Y,Yin W,Yang Q,Ren J,Tan L,Wang J,Liu J,Lu Q,Ding H,Zhan W,Hao L,Wang M

PA1-26204 was used in Immunohistochemistry (Paraffin) to investigate the immunological function of inhibiting cathepsin K in autophagy associated with peridontitis-aggravated rheumatoid arthritis.

Mon Nov 16 00:00:00 EST 2020

Role of sialidase Neu3 and ganglioside GM3 in cardiac fibroblasts activation.

The Biochemical journal

Ghiroldi A,Piccoli M,Creo P,Cirillo F,Rota P,D'Imperio S,Ciconte G,Monasky MM,Micaglio E,Garatti A,Aureli M,Carsana EV,Menicanti L,Pappone C,Anastasia L

PA1-26204 was used in Western Blot to identify pro-fibrotic pathways in primary cultures of human cardiac fibroblasts as targets for cardiac fibrosis.

Fri Sep 18 00:00:00 EDT 2020

NSM00158 Specifically Disrupts the CtBP2-p300 Interaction to Reverse CtBP2-Mediated Transrepression and Prevent the Occurrence of Nonunion.

Molecules and cells

Chen X,Zhang W,Zhang Q,Song T,Yu Z,Li Z,Duan N,Dang X

PA1-26204 was used in Western Blotting to suggest that NSM00158 represents a new potential compound to prevent the occurrence of nonunion by disrupting CtBP2 function and impairing the assembly of the CtBP2-p300-Runx2 transcriptional complex.

Tue Jun 30 00:00:00 EDT 2020

Generation of Vascularized Neural Organoids by Co-culturing with Mesodermal Progenitor Cells.

STAR protocols

Wörsdörfer P,Rockel A,Alt Y,Kern A,Ergün S

PA1-26204 was used in Immunohistochemistry-immunofluorescence to describe a protocol to incorporate stromal components, first of all blood vessels, by co-culturing with induced pluripotent stem cell-derived mesodermal progenitor cells.

Fri Jun 19 00:00:00 EDT 2020

YBX-1 mediated sorting of miR-133 into hypoxia/reoxygenation-induced EPC-derived exosomes to increase fibroblast angiogenesis and MEndoT.

Stem cell research & therapy

Lin F,Zeng Z,Song Y,Li L,Wu Z,Zhang X,Li Z,Ke X,Hu X

Published figure using Collagen I polyclonal antibody (Product # PA1-26204) in Western Blot

Fri Aug 23 00:00:00 EDT 2019

Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity.

Scientific reports

Buken C,Sahana J,Corydon TJ,Melnik D,Bauer J,Wehland M,Krüger M,Balk S,Abuagela N,Infanger M,Grimm D

PA1-26204 was used in Flow cytometry/Cell sorting to show simulated microgravity causes changes in the cytoskeleton, extracellular matrix, focal adhesion and growth behaviour of normal human dermal fibroblasts and to identify the factors involved in the assembly of the fibroblasts.

Thu Aug 15 00:00:00 EDT 2019

Fully Three-Dimensional Bioprinted Skin Equivalent Constructs with Validated Morphology and Barrier Function.

Tissue engineering. Part C, Methods

Derr K,Zou J,Luo K,Song MJ,Sittampalam GS,Zhou C,Michael S,Ferrer M,Derr P

Published figure using Collagen I polyclonal antibody (Product # PA1-26204) in Immunohistochemistry

Sat Jun 01 00:00:00 EDT 2019

Anti-inflammatory and anti-fibrotic profile of fish oil emulsions used in parenteral nutrition-associated liver disease.

PloS one

Pastor-Clerigues A,Marti-Bonmati E,Milara J,Almudever P,Cortijo J

PA1-26204 was used in immunohistochemistry - paraffin section to investigate the effect of soybean and fish oil lipid commercial emulsions on inflammatory and profibrotic liver markers in adults with parenteral nutrition long term associate liver disease

Wed Mar 09 00:00:00 EST 2016

Anti-inflammatory and anti-fibrotic profile of fish oil emulsions used in parenteral nutrition-associated liver disease.

PloS one

Pastor-Clerigues A,Marti-Bonmati E,Milara J,Almudever P,Cortijo J

Wed Mar 09 00:00:00 EST 2016

Simvastatin Increases the Ability of Roflumilast N-oxide to Inhibit Cigarette Smoke-Induced Epithelial to Mesenchymal Transition in Well-differentiated Human Bronchial Epithelial Cells in vitro.

COPD

Milara J,Peiró T,Serrano A,Artigues E,Aparicio J,Tenor H,Sanz C,Cortijo J

PA1-26204 was used in immunocytochemistry and western blot to discuss drugs that prevent the epithelial-mesenchymal transition.

Mon Jun 01 00:00:00 EDT 2015

Simvastatin Increases the Ability of Roflumilast N-oxide to Inhibit Cigarette Smoke-Induced Epithelial to Mesenchymal Transition in Well-differentiated Human Bronchial Epithelial Cells in vitro.

COPD

Milara J,Peiró T,Serrano A,Artigues E,Aparicio J,Tenor H,Sanz C,Cortijo J

PA1-26204 was used in immunocytochemistry and western blot to discuss drugs that prevent the epithelial-mesenchymal transition.

Mon Jun 01 00:00:00 EDT 2015

Roflumilast N-oxide inhibits bronchial epithelial to mesenchymal transition induced by cigarette smoke in smokers with COPD.

Pulmonary pharmacology & therapeutics

Milara J,Peiró T,Serrano A,Guijarro R,Zaragozá C,Tenor H,Cortijo J

PA1-26204 was used in Reverse phase protein array to study the epithelial to mesenchymal transition in the bronchial epithelium of patients with COPD and smokers

Fri Aug 01 00:00:00 EDT 2014

Calcimimetics increase CaSR expression and reduce mineralization in vascular smooth muscle cells: mechanisms of action.

Cardiovascular research

Hénaut L,Boudot C,Massy ZA,Lopez-Fernandez I,Dupont S,Mary A,Drüeke TB,Kamel S,Brazier M,Mentaverri R

PA1-26204 was used in immunohistochemistry and western blot to study the the role of increased CaSr expression in the mechanism by which calcimimetics reduce vascular smooth muscle cell mineralization

Sat Feb 01 00:00:00 EST 2014

miR-150 promotes renal fibrosis in lupus nephritis by downregulating SOCS1.

Journal of the American Society of Nephrology : JASN

Zhou H,Hasni SA,Perez P,Tandon M,Jang SI,Zheng C,Kopp JB,Austin H,Balow JE,Alevizos I,Illei GG

PA1-26204 was used in immunocytochemistry and western blot to study the role of SOCS1 downregulation in the mechanism by which miRNA-150 promotes renal fibrosis in patients with lupus nephritis

Sat Jun 01 00:00:00 EDT 2013

Epithelial to mesenchymal transition is increased in patients with COPD and induced by cigarette smoke.

Thorax

Milara J,Peiró T,Serrano A,Cortijo J

PA1-26204 was used in immunohistochemistry to study the role of smoking and COPD in promoting epithelial-to-mesenchymal transition in small airways

Wed May 01 00:00:00 EDT 2013

Aclidinium inhibits human lung fibroblast to myofibroblast transition.

Thorax

Milara J,Serrano A,Peiró T,Gavaldà A,Miralpeix M,Morcillo EJ,Cortijo J

Published figure using Collagen I polyclonal antibody (Product # PA1-26204) in Western Blot

Thu Mar 01 00:00:00 EST 2012

Vitamin D inhibits proliferation and profibrotic marker expression in hepatic stellate cells and decreases thioacetamide-induced liver fibrosis in rats.

Gut

Abramovitch S,Dahan-Bachar L,Sharvit E,Weisman Y,Ben Tov A,Brazowski E,Reif S

PA1-26204 was used in western blot to study the role of vitamin D in hepatic stellate cell proliferation and liver fibrosis

Thu Dec 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha-1 type 1 collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; alpha1(I) procollagen; COL1; collagen 1 alpha 2 chain; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen COL1A2; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; osteogenesis imperfecta; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; type I-alpha 1 collagen

Gene Aliases: AA960264; AI325291; Col1a-1; Col1a-2; COL1A1; COL1A2; Cola-1; Cola-2; Cola1; Cola2; COLIA1; EDSC; Mov-13; Mov13; OI1; OI2; OI3; OI4; oim

UniProt ID: (Human) Q15201, (Bovine) P02453, (Human) P08123, (Bovine) P02465, (Rat) P02454, (Mouse) P11087, (Mouse) Q01149, (Rat) P02466

Entrez Gene ID: (Human) 1277, (Bovine) 282187, (Human) 1278, (Bovine) 282188, (Rat) 29393, (Mouse) 12842, (Mouse) 12843, (Rat) 84352

Molecular Function: extracellular matrix structural protein