Invitrogen

Collagen I Polyclonal Antibody

Product Image Gallery

Collagen I Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of Collagen I was performed in formalin fixed, paraffin embedded normal kidney using Collagen I Polyclonal Antibody (Product # PA1-26204) at a dilution of 1:100 for 4 hours at room temperature. A peroxidase goat anti-rabbit was used as a secondary antibody at 1:10,000 for 45 min at RT. Localization: Distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain. Antigen retrieval: No antigen retrieval was performed.

Collagen I Antibody in Western Blot (WB) — Western blot analysis of Human Collagen Type I was performed by loading 50 ng of Human Collagen Type 1 onto a membrane and blotting with a Collagen I Polyclonal Antibody (Product # PA1-26204) at a dilution of 1:1,000 overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 139 & 130 kDa.

Collagen I Antibody — Figure 2 Collagen I deposition, localization, and quantification in decellularized scaffolds of HDF and JIMT-1/HDF 3D cultures. ( A - P ) Single confocal plane images of collagen I deposition taken in the centre of decellularized 3D cultures incubated in the absence (control) or presence of 5 ng/ml TGF-beta1. The 3D cultures were decellularized, fixed in 3.7% formaldehyde and stained to visualize fibronectin (green) at 3, 7, 10, and 14 days of culturing. All images are representative of 3 independent experiments. Scale bar is 50 mum. Quantification of fluorescence intensity using ImageJ in images taken by epifluorescence microscopy in HDF decellularized scaffolds ( Q ) and JIMT-1/HDF decellularized scaffolds ( R ). The columns are represented as mean +- SEM (n = 3).

Collagen I Antibody in Immunocytochemistry (ICC/IF) — Figure 9 Omegaven(r) inhibits myofibroblast markers induced by TGFbeta1. Human liver epithelial cell line THLE-3 was incubated in presence or absence of lipid emulsions Omegaven(r) 10%, Lipofundin MCT/LCT(r) 20%, ClinOleic(r) 20% or SMOFlipid(r) 20% at different dilutions, for 30 min followed by TGFbeta1 5 ng/mL stimulation for additional 72 hours. (A) Visible morphology and immunofluorescence for alpha smooth muscle actin (alphaSMA) and collagen type I (col type I) distribution and expression. B) Expression of mRNA of alphaSMA and col type I . Scale bar: 10 um. Results are expressed as means +- SEM of six independent experiments. * p <0.05 related to the control group. # p <0.05 related to the stimulus.

Collagen I Antibody in Immunohistochemistry (IHC) — FIG. 4. IHC comparison of protein expression between bioprinted tissues and native human skin show comparable morphology. Tight junction proteins ZO-1, Claudin I, and E-Cadherin. Epidermal differentiation proteins Keratin 1, Filaggrin, and Cytokeratin 15. ECM proteins Collagen I and Collagen VII and cell proliferation marker Ki67. Scale bar, 100 mum. ECM, extracellular matrix; IHC, immunohistochemistry.

Collagen I Antibody in Flow Cytometry (Flow) — Figure 7 Alterations in collagen levels of juvenile NDHF cells following a 3-day exposure to microgravity: Immunofluorescence images of 1 g -control cells, RPM-AD and RPM-MCS of collagen-I ( A - C ) and collagen-IV ( D - F ). Transcriptional and translational collagen type I analysis: Quantitative gene expression levels of COL1A1 ( G ), intracellular collagen-I levels ( H ) and flow cytometric analysis of collagen type I-labeled cells ( I ). Quantitative gene expression levels of COL4A5 ( J ), intracellular collagen-I levels ( K ) and flow cytometric analysis of fibronectin-labeled cells ( L ). Flow cytometric analysis of collagen type III-labeled cells ( M ). Quantitative gene expression levels of TIMP1 ( N ), MMP1 ( O ), MMP3 ( P ) and MMP14 ( Q ). Full-length blots of cropped Western blot images are presented in Supplementary Fig. S6 . *p < 0.05 1 g vs. RPM; # p < 0.05 AD vs. MCS. Scale bars: 50 um.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

1:5,000-1:50,000

Immunoprecipitation (IP)

1:100

Dot blot (DB)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Collagen type I extracted and purified from human and bovine placenta by limited pepsin digestion and selective salt precipitation.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.125M sodium borate, pH 8.0, with 5mM EDTA, 0.075M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2260734

Product Specific Information

Some class-specific anti-collagens are specific for three-dimensional epitopes, resulting in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Cross-reactivity with other human serum proteins and non-collagen extracellular matrix proteins is neglible.|Western blot: this preparation results in a native conformation of the protein. It is recommend not to add 2-ME or DTT in lysis buffer and not boiling the sample.

Target Information

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha-1 type 1 collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; alpha1(I) procollagen; COL1; collagen 1 alpha 2 chain; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen COL1A2; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; osteogenesis imperfecta; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; type I-alpha 1 collagen

Gene Aliases: AA960264; AI325291; Col1a-1; Col1a-2; COL1A1; COL1A2; Cola-1; Cola-2; Cola1; Cola2; COLIA1; EDSC; Mov-13; Mov13; OI1; OI2; OI3; OI4; oim

UniProt ID: (Human) P02452, (Bovine) P02453, (Human) P08123, (Bovine) P02465, (Rat) P02454, (Mouse) P11087, (Mouse) Q01149, (Rat) P02466

Entrez Gene ID: (Human) 1277, (Bovine) 282187, (Human) 1278, (Bovine) 282188, (Rat) 29393, (Mouse) 12842, (Mouse) 12843, (Rat) 84352

Molecular Function: extracellular matrix structural protein