Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000-1:10,000 | View 10 publications 10 publications |
Immunohistochemistry (IHC) |
- | View 5 publications 5 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:200 | View 2 publications 2 publications |
Immunocytochemistry (ICC/IF) |
- | View 4 publications 4 publications |
Flow Cytometry (Flow) |
- | View 1 publication 1 publication |
ELISA (ELISA) |
1:5,000-1:50,000 | - |
Immunoprecipitation (IP) |
1:100 | - |
Dot blot (DB) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Bovine, Human, Mouse, Rat |
Published species |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Collagen type I extracted and purified from human and bovine placenta by limited pepsin digestion and selective salt precipitation. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1.15 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
0.125M sodium borate, pH 8.0, with 5mM EDTA, 0.075M NaCl |
Contains |
0.01% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2260734 |
Some class-specific anti-collagens are specific for three-dimensional epitopes, resulting in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Cross-reactivity with other human serum proteins and non-collagen extracellular matrix proteins is neglible.|Western blot: this preparation results in a native conformation of the protein. It is recommend not to add 2-ME or DTT in lysis buffer and not boiling the sample.
Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha-1 type 1 collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; alpha1(I) procollagen; COL1; collagen 1 alpha 2 chain; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen COL1A2; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; osteogenesis imperfecta; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; type I-alpha 1 collagen
Gene Aliases: AA960264; AI325291; Col1a-1; Col1a-2; COL1A1; COL1A2; Cola-1; Cola-2; Cola1; Cola2; COLIA1; EDSC; Mov-13; Mov13; OI1; OI2; OI3; OI4; oim
UniProt ID: (Human) Q15201, (Bovine) P02453, (Human) P08123, (Bovine) P02465, (Rat) P02454, (Mouse) P11087, (Mouse) Q01149, (Rat) P02466
Entrez Gene ID: (Human) 1277, (Bovine) 282187, (Human) 1278, (Bovine) 282188, (Rat) 29393, (Mouse) 12842, (Mouse) 12843, (Rat) 84352
Molecular Function:
extracellular matrix structural protein
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