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Invitrogen
Collagen II Polyclonal Antibody
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FIGURE: 1 / 1
Collagen II Antibody (PA5-95138) in WB
Western blot analysis of Collagen II in Lane 1: COLO320 whole cell lysate (40 µg), Lane 2: HeLa whole cell lysate (40 µg), Lane 3: MCF-7whole cell lysate (40 µg), Lane 4: A549 whole cell lysate (40 µg), Lane 5: Jurkat whole cell lysate (40 µg). Samples were incubated with Collagen II polyclonal antibody (Product # PA5-95138).
Product Details
PA5-95138
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen II (1222-1241aa AFAGLGPREKGPDPLQYMRA).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: COLO320 whole cell, HELA whole cell, MCF-7whole cell, A549 whole cell, JURKAT whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
COL2A1 is the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-1 type II collagen; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; CGA; chondrocalcin; Collagen 2; Collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen type II (Col2A1) gene, enhancer region; collagen, type II, alpha 1; disproportionate micromelia; MGC131516; Procollagen II alpha 1; procollagen, type II, alpha 1
Gene Aliases: ANFH; AOM; CG2A1A; COL11A3; Col2; Col2a; Col2a-1; COL2A1; COLLII; Del1; Dmm; Lpk; M100856; Rgsc856; SEDC; STL1
UniProt ID: (Human) P02458, (Rat) P05539, (Mouse) P28481
Entrez Gene ID: (Human) 1280, (Rat) 25412, (Mouse) 12824
skeletal system development
cartilage condensation
tissue homeostasis
endochondral ossification
chondrocyte differentiation
heart morphogenesis
proteoglycan metabolic process
central nervous system development
visual perception
sensory perception of sound
regulation of gene expression
extracellular matrix organization
collagen fibril organization
collagen catabolic process
notochord development
inner ear morphogenesis
regulation of immune response
cartilage development
palate development
limb bud formation
embryonic skeletal joint morphogenesis
cartilage development involved in endochondral bone morphogenesis
otic vesicle development
cellular response to BMP stimulus
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
growth plate cartilage development
response to mechanical stimulus
response to X-ray
limb morphogenesis
skeletal system morphogenesis
inner ear development
bone development
cellular response to mechanical stimulus
cellular response to retinoic acid
cellular response to vitamin E
cellular response to nicotine
cellular response to tumor necrosis factor
cellular response to parathyroid hormone stimulus
cellular response to peptide hormone stimulus
response to fibroblast growth factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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