Invitrogen

Collagen II Polyclonal Antibody

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Collagen II Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Collagen II in human colon cancer tissue. Samples were incubated with Collagen II polyclonal antibody (Product # PA5-99159) using a dilution of 1:100.

Collagen II Antibody in Western Blot (WB) — Western blot analysis of Collagen II in COLO205 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with Collagen II polyclonal antibody (Product # PA5-99159).

Product Details

PA5-99159

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human Collagen II, corresponding to a region within N-terminal amino acids.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2818092

Target Information

COL2A1 is the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Caffeic acid phenethyl ester attenuates osteoarthritis progression by activating NRF2/HO‑1 and inhibiting the NF‑κB signaling pathway.

International journal of molecular medicine

Sun W,Xie W,Huang D,Cui Y,Yue J,He Q,Jiang L,Xiong J,Sun W,Yi Q

PA5-99159 was used in Western Blot, Immunohistochemistry to investigate the antiinflammatory effects of CAPE on IL‚1β‚stimulated chondrocytes in vitro and surgically induced rat models of OA in vivo.

Tue Nov 01 00:00:00 EDT 2022

Caffeic acid phenethyl ester attenuates osteoarthritis progression by activating NRF2/HO‑1 and inhibiting the NF‑κB signaling pathway.

International journal of molecular medicine

Sun W,Xie W,Huang D,Cui Y,Yue J,He Q,Jiang L,Xiong J,Sun W,Yi Q

Tue Nov 01 00:00:00 EDT 2022

Pharmacological Disruption of Phosphorylated Eukaryotic Initiation Factor-2α/Activating Transcription Factor 4/Indian Hedgehog Protects Intervertebral Disc Degeneration via Reducing the Reactive Oxygen Species and Apoptosis of Nucleus Pulposus Cells.

Frontiers in cell and developmental biology

Bao J,Qian Z,Liu L,Hong X,Che H,Wu X

PA5-99159 was used in Immunohistochemistry to investigate the impact of phosphorylation of eukaryotic initiation factor-2α (eIF2α)/activating transcription factor 4 (ATF4)/Indian hedgehog (Ihh) signalling in intervertebral disc degeneration.

Fri Jun 25 00:00:00 EDT 2021

Nanoscale Thermosensitive Hydrogel Scaffolds Promote the Chondrogenic Differentiation of Dental Pulp Stem and Progenitor Cells: A Minimally Invasive Approach for Cartilage Regeneration.

International journal of nanomedicine

Talaat W,Aryal Ac S,Al Kawas S,Samsudin ABR,Kandile NG,Harding DRK,Ghoneim MM,Zeiada W,Jagal J,Aboelnaga A,Haider M

PA5-99159 was used in Immunohistochemistry to prepare nanocellulose-based thermosensitive injectable hydrogel scaffolds and assess their potential as 3D scaffolds allowing the chondrogenic differentiation of embedded human dental pulp stem and progenitor cells (hDPSCs).

Fri Nov 20 00:00:00 EST 2020

Bioinformatics

Protein Aliases: Alpha-1 type II collagen; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; CGA; Chondrocalcin; Collagen 2; Collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen type II (Col2A1) gene, enhancer region; collagen, type II, alpha 1; disproportionate micromelia; MGC131516; Procollagen II alpha 1; procollagen, type II, alpha 1

Gene Aliases: ANFH; AOM; CG2A1A; COL11A3; Col2; Col2a; Col2a-1; COL2A1; COLLII; Del1; Dmm; Lpk; M100856; Rgsc856; SEDC; STL1

UniProt ID: (Human) P02458, (Rat) P05539, (Mouse) P28481

Entrez Gene ID: (Human) 1280, (Rat) 25412, (Mouse) 12824

Molecular Function: extracellular matrix structural protein