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| Product Specifications | |
|---|---|
Species Reactivity |
Bovine, Human, Pig, Rat |
Host/Isotype |
Rabbit / Ig |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Collagen IV |
Conjugate |
Unconjugated |
Form |
Liquid |
Storage buffer |
whole serum |
Contains |
0.09% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
For immunohistochemistry on paraffin tissue the sections should be pretreated with 1 mg/mL pepsin in 0.01 N HCl for 30 minutes at room temperature.
The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. Collagen IV is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. Collagen IV can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alpha 1 type IV collagen; alpha-2 collagen type IV; alpha-3 type IV collagen; Arresten; Canstatin; COL4A1 NC1 domain; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen IV, alpha-5 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen of basement membrane, alpha-5 chain; collagen, type IV, alpha 1; collagen, type IV, alpha 2; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; collagen, type IV, alpha 5; dA149D17.3; dA24A23.1; Goodpasture antigen; procollagen, type IV, alpha 1; procollagen, type IV, alpha 2; procollagen, type IV, alpha 3; procollagen, type IV, alpha 4; procollagen, type IV, alpha 5; Tumstatin; Type IV Collagen
Gene Aliases: ASLN; ATS; BSVD; CA44; CA54; COL4A1; COL4A2; COL4A3; COL4A4; COL4A5; ICH; POREN2; RATOR; RGD1565499
UniProt ID: (Human) P02462, (Human) P08572, (Human) Q9BQT2, (Human) P53420, (Human) P29400
Entrez Gene ID: (Pig) 100515336, (Human) 1282, (Bovine) 282191, (Human) 1284, (Pig) 100153454, (Human) 1285, (Bovine) 317711, (Pig) 100621504, (Human) 1286, (Bovine) 407107, (Pig) 100621403, (Pig) 100737091, (Human) 1287, (Rat) 290905, (Rat) 306628, (Rat) 363265, (Rat) 301562, (Rat) 363457
Molecular Function:
extracellular matrix structural protein
extracellular matrix protein
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