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Invitrogen
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:1,000-1:10,000 | - |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:200 | View 1 publication 1 publication |
ELISA (ELISA) |
1:5,000-1:50,000 | - |
Immunoprecipitation (IP) |
1:100 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Bovine, Human, Mammal, Mouse, Rat |
Published species |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Collagen Type IV from human and bovine placenta |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1.17 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
Contains |
0.01% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_1956972 |
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. Collagen IV is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. Collagen IV can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alpha 1 type IV collagen; alpha-2 collagen type IV; alpha1(IV) collagen; Arresten; Canstatin; COL4A1 NC1 domain; col4a3; col4a4; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen type IV alpha3 chain; collagen, type IV, alpha 1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; Del(8)Bru44H; Goodpasture antigen; procollagen, type IV, alpha 1; procollagen, type IV, alpha 2; procollagen, type IV, alpha 3; procollagen, type IV, alpha 4; retinal anterior wiring; Tumstatin; Type IV Collagen
Gene Aliases: [a]3(IV); [a]4(IV); alpha3(IV); Bru; BSVD; CA44; Col4a-1; Col4a-2; COL4A1; COL4A2; COL4A3; COL4A4; Del(8)44H; E130010M05Rik; ICH; POREN2; RATOR; Raw; Svc
UniProt ID: (Human) P02462, (Human) P08572, (Human) Q01955, (Human) P53420, (Mouse) P02463, (Mouse) P08122, (Mouse) Q9QZS0, (Mouse) Q9QZR9
Entrez Gene ID: (Human) 1282, (Bovine) 282191, (Human) 1284, (Human) 1285, (Bovine) 317711, (Human) 1286, (Bovine) 407107, (Rat) 290905, (Mouse) 12826, (Rat) 306628, (Mouse) 12827, (Rat) 363265, (Mouse) 12828, (Mouse) 12829, (Rat) 301562
Molecular Function:
extracellular matrix structural protein
extracellular matrix protein
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