Invitrogen

Collagen IV Polyclonal Antibody

Product Image Gallery

Collagen IV Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Collagen IV was performed in human kidney, glomeruli and liver tissues using Collagen IV Polyclonal Antibody (Product # PA1-28534) at a dilution of 1:400 for 45 minutes a room temperature. The antibody showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.

Collagen IV Antibody — Figure 12 TCP 100x collagen IV stain showing moderate fibrosis.

Product Details

Applications

Tested Dilution

Publications

ELISA (ELISA)

1:5000-1:50000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunoprecipitation (IP)

1:100

Western Blot (WB)

1:1,000-1:10,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Product Specifications

Species

Bovine, Human, Mammal, Mouse, Rat

Published species

Not Applicable

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Collagen Type IV from human and bovine placenta

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_1956972

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Collagen IV is a major constituent of the basement membranes along with laminins and enactins. Collagen IV is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. Collagen IV can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha 1 type IV collagen; alpha-2 collagen type IV; alpha1(IV) collagen; Arresten; Canstatin; COL4A1 NC1 domain; col4a3; col4a4; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen type IV alpha3 chain; collagen, type IV, alpha 1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; Del(8)Bru44H; Goodpasture antigen; procollagen, type IV, alpha 1; procollagen, type IV, alpha 2; procollagen, type IV, alpha 3; procollagen, type IV, alpha 4; retinal anterior wiring; Tumstatin; Type IV Collagen

Gene Aliases: [a]3(IV); [a]4(IV); alpha3(IV); Bru; BSVD; CA44; Col4a-1; Col4a-2; COL4A1; COL4A2; COL4A3; COL4A4; Del(8)44H; E130010M05Rik; ICH; POREN2; RATOR; Raw; Svc

UniProt ID: (Human) P02462, (Human) P08572, (Human) Q01955, (Human) P53420, (Mouse) P02463, (Mouse) P08122, (Mouse) Q9QZS0, (Mouse) Q9QZR9

Entrez Gene ID: (Human) 1282, (Bovine) 282191, (Human) 1284, (Human) 1285, (Bovine) 317711, (Human) 1286, (Bovine) 407107, (Rat) 290905, (Mouse) 12826, (Rat) 306628, (Mouse) 12827, (Rat) 363265, (Mouse) 12828, (Mouse) 12829, (Rat) 301562