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Immunohistochemical analysis of Collagen IV in formalin-fixed, paraffin-embedded human kidney (left) and glomeruli and liver (right) with strong staining in sinusoids using a Collagen IV polyclonal antibody (Product # PA1-28534) at a dilution of 1:400 for 45 min at room temperature. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0, at 99-100°C for 20 minutes for antigen retrieval.
|Tested species reactivity||Cattle , Human , Mammals|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Collagen Type IV from human and bovine placenta|
|Purification||Antigen affinity chromatography|
|Storage buffer||BBS, pH 8.0, with 0.005M EDTA|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:5000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28534 detects Collagen IV from bovine, human, and mammal samples.
PA1-28534 has been successfully used in ELISA, immunohistochemistry (paraffin), immunoprecipitation, and Western applications.
The PA1-28534 immunogen is: Collagen Type IV from human and bovine placenta
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is usefu
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
alpha1(IV) collagen; arresten; COL4A1 NC1 domain; collagen alpha-1(IV) chain; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain; collagen, type IV, alpha 1; Del(8)Bru44H; procollagen, type IV, alpha 1; retinal anterior wiring
BOS_12713; Bru; BSVD; Col4a-1; COL4A1; Del(8)44H; RATOR; Raw; Svc