Invitrogen

Collagen IV Polyclonal Antibody

Product Image Gallery

Collagen IV Antibody — Figure 12 TCP 100x collagen IV stain showing moderate fibrosis.

Collagen IV Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Collagen IV in formalin-fixed, paraffin-embedded human kidney (left) and glomeruli and liver (right) with strong staining in sinusoids using a Collagen IV polyclonal antibody (Product # PA1-28534) at a dilution of 1:400 for 45 min at room temperature. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0, at 99-100°C for 20 minutes for antigen retrieval.

Product Details

Tested Applications

Dilution

ELISA (ELISA)

1:5000-1:50000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunoprecipitation (IP)

1:100

Western Blot (WB)

1:5000-1:10,000

Published Applications

Immunohistochemistry (IHC)

See 1 publication below

Product Specifications

Species Reactivity

Bovine, Human, Mammal

Published species

Not Applicable

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Collagen Type IV from human and bovine placenta

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.125M sodium borate, pH 8.0, with 0.005M EDTA

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_1956972

Product Specific Information

PA1-28534 detects Collagen IV from bovine, human, and mammal samples.

PA1-28534 has been successfully used in ELISA, immunohistochemistry (paraffin), immunoprecipitation, and Western applications.

The PA1-28534 immunogen is: Collagen Type IV from human and bovine placenta

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

Collagen IV is a major constituent of the basement membranes along with laminins and enactins. Collagen IV is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. Collagen IV can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha 1 type IV collagen; alpha-2 collagen type IV; alpha1(IV) collagen; Arresten; Canstatin; COL4A1 NC1 domain; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain; collagen, type IV, alpha 1; Del(8)Bru44H; procollagen, type IV, alpha 1; procollagen, type IV, alpha 2; retinal anterior wiring

Gene Aliases: BOS_12713; Bru; BSVD; Col4a-1; Col4a-2; COL4A1; COL4A2; Del(8)44H; ICH; POREN2; RATOR; Raw; Svc

UniProt ID: (Human) P02462, (Human) P08572, (Mouse) P02463, (Mouse) P08122

Entrez Gene ID: (Human) 1282, (Bovine) 282191, (Human) 1284, (Rat) 290905, (Mouse) 12826, (Rat) 306628, (Mouse) 12827