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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:2,000-1:10,000 | - |
Immunohistochemistry (IHC) |
1:1,000-1:5,000 | - |
ELISA (ELISA) |
1:20,000-1:100,000 | - |
Immunoprecipitation (IP) |
1:100 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Bovine, Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta. |
Conjugate |
FITC |
Excitation/Emission Profile |
View spectra
|
Form |
Lyophilized |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
0.01M sodium phosphate, pH 7.2, with 0.25M NaCl, 10mg/mL BSA |
Contains |
0.01% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. Collagen IV is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. Collagen IV can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Arresten; Canstatin; COL4A1 NC1 domain; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen, type IV, alpha 1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; Goodpasture antigen; Tumstatin; Type IV Collagen
Gene Aliases: BSVD; CA44; COL4A1; COL4A2; COL4A3; COL4A4; ICH; POREN2; RATOR
UniProt ID: (Human) P02462, (Human) P08572, (Human) Q01955, (Human) P53420
Entrez Gene ID: (Human) 1282, (Bovine) 282191, (Human) 1284, (Human) 1285, (Bovine) 317711, (Human) 1286, (Bovine) 407107
Molecular Function:
extracellular matrix structural protein
extracellular matrix protein
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