Search
Rockland
Collagen Type IV Polyclonal Antibody, HRP
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
Collagen Type IV Antibody (600-403-106) in IHC (P)
Immunohistochemistry of Rabbit Anti-Collagen IV Biotin Conjugated Antibody. Tissue: human kidney (Left) with strong red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Fixation: formalin fixed paraffin embedded. Antigen retrieval: steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Primary antibody: Collagen IV antibody at 10 µg/mL for 1 h at RT. ... View More
Product Details
600-403-106
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 10mg/mL BSA
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Target Information
Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: a matrix-derived inhibitor of angiogenesis and tumor growth; a3(IV)NC1; arresten; canstatin; COL4A1 NC1 domain; COL4A2 NC1 domain; COL4A3 NC1 domain; Collagen 4; Collagen alpha-1(IV) chain; collagen alpha-2 type IV; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen IV, alpha-5 polypeptide; collagen IV, alpha-6 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen of basement membrane, alpha-5 chain; collagen of basement membrane, alpha-6; collagen type IV alpha 5 chain; collagen, type IV, alpha 1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; collagen, type IV, alpha 5; collagen, type IV, alpha 6; contains COL4A1 NC1 domain; dA149D17.3; dA24A23.1; dJ889N15.4 (Collagen Alpha 6(IV)); Goodpasture antigen; matrix-derived inhibitor of angiogenesis and tumor growth; non-collagenous 1 domain from alpha 1 chain of type IV collagen; pro-alpha-1(IV); procollagen alpha-1 type IV; soluble Col4A1 protein; structural non-collagenous NC1 domain; a6[IV]NC1; tumstatin; Type IV Collagen; unnamed protein product
Gene Aliases: ASLN; ATS; ATS1; ATS2; ATS3; ATS3A; ATS3B; BFH; BFH1; BFH2; BSVD; BSVD1; BSVD2; CA44; CA54; COL4A1; COL4A1s; COL4A2; COL4A3; COL4A4; COL4A5; COL4A6; CXDELq22.3; DELXq22.3; DFNX6; ICH; PADMAL; POREN2; RATOR
UniProt ID: (Human) P02462, (Bovine) Q7SIB2, (Human) P08572, (Human) Q01955, (Human) P53420, (Human) P29400, (Human) Q14031
Entrez Gene ID: (Human) 1282, (Bovine) 282191, (Human) 1284, (Bovine) 317711, (Human) 1285, (Bovine) 407107, (Human) 1286, (Human) 1287, (Human) 1288
extracellular matrix structural constituent
protein binding
extracellular matrix structural constituent conferring tensile strength
platelet-derived growth factor binding
binding, bridging
integrin binding
structural molecule activity
metalloendopeptidase inhibitor activity
extracellular matrix structural protein
angiogenesis
patterning of blood vessels
brain development
neuromuscular junction development
epithelial cell differentiation
collagen-activated tyrosine kinase receptor signaling pathway
blood vessel morphogenesis
retinal blood vessel morphogenesis
renal tubule morphogenesis
cellular response to amino acid stimulus
basement membrane organization
transcription, DNA-templated
response to activity
negative regulation of angiogenesis
extracellular matrix organization
endodermal cell differentiation
cellular response to transforming growth factor beta stimulus
cell adhesion
cell surface receptor signaling pathway
sensory perception of sound
negative regulation of cell proliferation
glomerular basement membrane development
endothelial cell apoptotic process
negative regulation of vascular endothelial cell proliferation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support