Proteintech

Collagen Type VIII Polyclonal Antibody

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Collagen Type VIII Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells using 17251-1-AP (Collagen Type VIII antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Collagen Type VIII Antibody in Western Blot (WB) — Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17251-1-AP (Collagen Type VIII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Collagen Type VIII Antibody in Immunoprecipitation (IP) — IP result of anti-Collagen Type VIII (IP:17251-1-AP, 4ug; Detection:17251-1-AP 1:500) with HepG2 cells lysate 1800ug.

Product Details

17251-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	1:20-1:200	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Dog, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Collagen Type VIII Fusion Protein Ag10607 (396-744 aa encoded by BC013581) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.29 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: GIPGPMGPP GAIGFPGPKG EGGIVGPQGP PGPKGEPGLQ GFPGKPGFLG EVGPPGMRGL PGPIGPKGEA GQKGVPGLPG VPGLLGPKGE PGIPGDQGLQ GPPGIPGIGG PSGPIGPPGI PGPKGEPGLP GPPGFPGIGK PGVAGLHGPP GKPGALGPQG QPGLPGPPGP PGPPGPPAVM PPTPPPQGEY LPDMGLGIDG VKPPHAYGAK KGKNGGPAYE MPAFTAELTA PFPPVGAPVK FNKLLYNGRQ NYNPQTGIFT CEVPGVYYFA YHVHCKGGNV WVALFKNNEP VMYTYDEYKK GFLDQASGSA VLLLRPGDRV FLQMPSEQAA GLYAGQYVHS SFSGYLLYPM (396-744 aa encoded by BC013581)

Target Information

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Effects of COL8A1 on the proliferation of muscle-derived satellite cells.

Cell biology international

Li X,Wang Z,Tong H,Yan Y,Li S

Sat Sep 01 00:00:00 EDT 2018

Upregulation of distinct collagen transcripts in post-surgery scar tissue: a study of conjunctival fibrosis.

Disease models & mechanisms

Seet LF,Toh LZ,Chu SWL,Finger SN,Chua JLL,Wong TT

Thu Jun 01 00:00:00 EDT 2017

Upregulation of distinct collagen transcripts in post-surgery scar tissue: a study of conjunctival fibrosis.

Disease models & mechanisms

Seet LF,Toh LZ,Chu SWL,Finger SN,Chua JLL,Wong TT

Thu Jun 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: cell proliferation-inducing protein 41; Collagen alpha-1(VIII) chain; collagen VIII, alpha-1 polypeptide; collagen, type VIII, alpha 1; Endothelial collagen; procollagen type VIII alpha 1; procollagen, type VIII, alpha 1; smag-64; smooth muscle cell-expressed and macrophage conditioned medium-induced protein smag-64; Vastatin

Gene Aliases: C3orf7; Col8a-1; COL8A1

UniProt ID: (Human) P27658, (Mouse) Q00780

Entrez Gene ID: (Human) 1295, (Mouse) 12837, (Rat) 304021

Molecular Function: extracellular matrix structural protein