Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.
In immunoelectrophoresis use 2 µL human plasma or equivalent against 120 µL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µL antiserum in 3 mm diameter center well and 2 µL plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion use 1 percent antiserum in the gel.
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day.
Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. C3 and C4, mainly produced by the liver, are the major plasma proteins of the immune system complement pathways. Measurements of serum complement components C3 and C4 are useful in the diagnosis and monitoring of immune complex disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: acidic C4; Acidic complement C4; Acylation stimulating protein; acylation-stimulating protein cleavage product; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; C3-beta-c; C3a anaphylatoxin; C3adesArg; C3bc; C4A anaphylatoxin; C4b-A; C4d-A; Complement C3; Complement C3 alpha chain; Complement C3 beta chain; Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3c alpha' chain fragment 2; Complement C3d fragment; Complement C3dg fragment; Complement C3f fragment; Complement C3g fragment; Complement C4 beta chain; Complement C4 gamma chain; Complement C4-A; Complement C4-A alpha chain; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; MHC class III region complement; prepro-C3; Rodgers form of C4
Gene Aliases: AHUS5; ARMD9; ASP; C3; C3a; C3b; C4; C4A; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4; CPAMD1; CPAMD2; HEL-S-62p; RG