Invitrogen

Complement C3 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Complement C3 Antibody in Immunocytochemistry (ICC/IF) — Figure 7. Assessment of the functional state of reactive astrocytes after TMT exposure. Double immunofluorescent staining of GFAP and IL-1beta, TNF-alpha, IL-10, C3, iNOS and NF-kB and corresponding integrated fluorescence density expressed as AUs +- SEM in the injured CA area at 7 and 21 dpi. Significance shown inside the graphs: * p < .05 or less compared to age-match Ctrl. Note . AU = arbitrary unit; Ctrl = control; dpi = days post intoxication; GFAP = glial fibrillary acidic protein; IL-10 = interleukin-10; IL-1beta = interleukin-1beta; iNOS = inducible nitric oxide synthase; NF-kB = nuclear factor-kB; SEM = standard error of the mean; TMT = trimethyltin; TNF-alpha = tumor necrosis factor alpha.

Complement C3 Antibody in Immunohistochemistry (IHC) — FIGURE 8 Heterogeneity of NTPDase1 positive astrocytes. Representative micrographs showing double immunofluorescence labeling directed to NTPDase1 ( red fluorescence ) and (A) glutamine synthetase - GS (B) vimentin, (C) Complement component 3 - C3, and (D) Cox-2 ( green fluorescence for all). Sections (A,B) were counterstained with Hoechst for cell nuclei ( blue fluorescence ). Sections (C,D) were co-labeled with GFAP ( blue fluorescence ). Scale bar applicable to all micrographs = 20 mum.

Complement C3 Antibody in Western Blot (WB) — Figure 4 Podocyte-secreted CFH functionally active. Functionality of secreted CFH was tested in a cofactor assay and in a C5b-9 deposition assay in podocytes with lost CFH derived complement control. (A) Cofactor assay: CFH together with CFI can split recombinant C3b. Split products with a size of 68 and 43 kDa can be detected in Western blot against C3. Podocyte supernatant with added CFI was able to cleave added recombinant C3b. Positive control: C3b, CFI and CFH were added to serum free media (SFM) in supersaturated amounts, leading to immediate cleavage of C3b (illustrated by the appearance of the cleavage products of alpha'68- and alpha'43 kD). There is no detection of any cleavage products after the incubation of C3b and CFI, or C3b and CFI in SFM (left part). When C3b and CFI are added to podocyte supernatant, C3b split products can be detected. (B) Podocyte lysate (HPC) shows a clear signal for CD46 as a membrane bound complement regulator, but did not shed CD46 into the supernatant. The treatment of podocytes with interferon gamma (IFNg, 10 ng/mL, 24 h) did not result in an induction of CD46 in the cell lysate or enhanced shedding in the cell culture supernatant (representative Western blot of 3 independent experiments). (C,D) The deposition of terminal complement complex C5b-9 was compared on normal human podocytes and podocytes from a patient suffering from atypical hemolytic uremic syndrome (aHUS). Patient's CFH was not able to bind to the cell surface. Complement

Product Details

PA1-29715

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:500

View 1 publication 1 publication

Flow Cytometry (Flow)

1:100

Immunoelectrophoresis (IE)

Assay-dependent

Product Specifications

Species Reactivity

Baboon, Horse, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Published species

Cynomolgus monkey, Guinea pig, Mouse, Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Human complement C3

Conjugate

Unconjugated

Form

Liquid

Concentration

9 mg/mL

Storage buffer

0.05M tris HCl, pH 7.5, with 500mM NaCl

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2066730

Product Specific Information

Specificity: Monospecific to human C3 complement component. This antibody has also been reported to recognize the C3 fragments, C3a, C3b and C3c.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A novel transgenic mouse model expressing primate-specific nuclear choline acetyltransferase: insights into potential cholinergic vulnerability.

Scientific reports

AlQot HE,Rylett RJ

PA1-29715 was used in Western Blotting to establish a novel transgenic mouse expressing 82-kDa ChAT that is valuable for studying the role of this primate-specific cholinergic enzyme in pathologies associated with cholinergic neuron vulnerability and dysfunction.

Tue Feb 21 00:00:00 EST 2023

Microglial- and Astrocyte-Specific Expression of Purinergic Signaling Components and Inflammatory Mediators in the Rat Hippocampus During Trimethyltin-Induced Neurodegeneration.

ASN neuro

Dragić M,Mitrović N,Adžić M,Nedeljković N,Grković I

PA1-29715 was used in Immunocytochemistry, Immunohistochemistry to investigated spatial and temporal patterns of ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase1/CD39) and ecto-5' nucleotidase (eN/CD73) activity, their cell-specific localization, and analyzed gene expression pattern and/or cellular localization of purinoreceptors and proinflammatory mediators associated with reactive glial cells.

Mon Jan 17 00:00:00 EST 2022

Microglial- and Astrocyte-Specific Expression of Purinergic Signaling Components and Inflammatory Mediators in the Rat Hippocampus During Trimethyltin-Induced Neurodegeneration.

ASN neuro

Dragić M,Mitrović N,Adžić M,Nedeljković N,Grković I

Mon Jan 17 00:00:00 EST 2022

Podocytes Produce and Secrete Functional Complement C3 and Complement Factor H.

Frontiers in immunology

Mühlig AK,Keir LS,Abt JC,Heidelbach HS,Horton R,Welsh GI,Meyer-Schwesinger C,Licht C,Coward RJ,Fester L,Saleem MA,Oh J

Published figure using Complement C3 polyclonal antibody (Product # PA1-29715) in Western Blot

Mon Apr 12 00:00:00 EDT 2021

Induction of NTPDase1/CD39 by Reactive Microglia and Macrophages Is Associated With the Functional State During EAE.

Frontiers in neuroscience

Jakovljevic M,Lavrnja I,Bozic I,Milosevic A,Bjelobaba I,Savic D,Sévigny J,Pekovic S,Nedeljkovic N,Laketa D

PA1-29715 was used in Immunohistochemistry to explore the expression of ectonucleoside triphosphate diphosphohydrolase1 (NTPDase1/CD39) in the spinal cord, at the onset (Eo), peak (Ep), and end (Ee) of experimental autoimmune encephalomyelitis (EAE).

Thu Oct 01 00:00:00 EDT 2020

Induction of NTPDase1/CD39 by Reactive Microglia and Macrophages Is Associated With the Functional State During EAE.

Frontiers in neuroscience

Jakovljevic M,Lavrnja I,Bozic I,Milosevic A,Bjelobaba I,Savic D,Sévigny J,Pekovic S,Nedeljkovic N,Laketa D

Thu Oct 01 00:00:00 EDT 2020

Prolonged intraocular residence and retinal tissue distribution of a fourth-generation compstatin-based C3 inhibitor in non-human primates.

Clinical immunology (Orlando, Fla.)

Hughes S,Gumas J,Lee R,Rumano M,Berger N,Gautam AK,Sfyroera G,Chan AL,Gnanaguru G,Connor KM,Kim BJ,Dunaief JL,Ricklin D,Hajishengallis G,Yancopoulou D,Reis ES,Mastellos DC,Lambris JD

PA1-29715 was used in Immunohistochemistry to report the intraocular distribution and pharmacokinetic profile of the fourth-generation compstatin analog, Cp40-KKK in cynomolgus monkeys following a single intravitreal injection.

Fri May 01 00:00:00 EDT 2020

The immunosuppression mechanism of hypodermin A on complement.

Parasitology international

Chen RJ,Hu AK,Yuan HH,Wang ZZ,Ji DJ,Wu LL,Zhang TY,Zhu YH,Sun W,Zhu XR

PA1-29715 was used in western blot to study the mechanism by which hypodermin A inhibits the complement pathway

Tue Apr 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Acylation stimulating protein; acylation-stimulating protein cleavage product; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3-beta-c; C3a anaphylatoxin; C3adesArg; C3bc; Complement C3; Complement C3 alpha chain; Complement C3 beta chain; complement C3 protein (GPC3) precursor; Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3c alpha' chain fragment 2; Complement C3d fragment; Complement C3dg fragment; Complement C3f fragment; Complement C3g fragment; complement component 3; complement component C3; complement component C3 alpha-chain; complement component C3a; complement component C3b; ENCF-1; ENCF-2; epididymis secretory sperm binding protein Li 62p; HSE-MSF; Neutrophil chemotactic factor-1; Neutrophil chemotactic factor-2; prepro-C3

Gene Aliases: AHUS5; AI255234; ARMD9; ASP; C3; C3a; C3b; C3d; CPAMD1; HEL-S-62p; HSE-MSF; I79_008227; Plp

UniProt ID: (Guinea pig) P12387, (Rat) P01026, (Rabbit) P12247, (Human) P01024, (Mouse) P01027, (Pig) P01025

Entrez Gene ID: (Guinea pig) 100135462, (Chinese hamster) 100763958, (Rat) 24232, (Rabbit) 100009121, (Human) 718, (Mouse) 12266, (Pig) 397072, (Horse) 100060539

Molecular Function: protease inhibitor