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Invitrogen
Complement C3 Polyclonal Antibody
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FIGURE: 1 / 4
Complement C3 Antibody (PA5-114921) in ICC/IF
Immunocytochemistry analysis of Complement C3 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114921) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated go... View More
Product Details
PA5-114921
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human C3(Accession P01024), corresponding to amino acid residues V944-A994.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total C3.
Target Information
C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Complement C3; complement component 3; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; HSE-MSF; prepro-C3; unnamed protein product
Gene Aliases: AHUS5; AI255234; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p; HSE-MSF; Plp
UniProt ID: (Human) P01024, (Rat) P01026, (Mouse) P01027
Entrez Gene ID: (Human) 718, (Rat) 24232, (Mouse) 12266
positive regulation of type IIa hypersensitivity
complement activation, lectin pathway
activation of membrane attack complex
positive regulation of protein phosphorylation
positive regulation of activation of membrane attack complex
immune system process
complement receptor mediated signaling pathway
lipid metabolic process
fatty acid metabolic process
inflammatory response
immune response
complement activation
complement activation, alternative pathway
complement activation, classical pathway
signal transduction
G-protein coupled receptor signaling pathway
opsonization
response to bacterium
positive regulation of vascular endothelial growth factor production
positive regulation of glucose transport
regulation of triglyceride biosynthetic process
positive regulation of lipid storage
neuron remodeling
leukocyte chemotaxis
killing of cells of other organism
oviduct epithelium development
B cell activation
innate immune response
positive regulation of G-protein coupled receptor protein signaling pathway
positive regulation of angiogenesis
positive regulation of receptor-mediated endocytosis
positive regulation of phagocytosis
protein maturation
positive regulation of phagocytosis, engulfment
beta-amyloid clearance
complement-dependent cytotoxicity
complement-mediated synapse pruning
vertebrate eye-specific patterning
complement activation, GZMK pathway
positive regulation of apoptotic cell clearance
tolerance induction
chemotaxis
blood coagulation
negative regulation of endopeptidase activity
response to magnesium ion
response to estradiol
response to progesterone
response to estrogen
positive regulation of developmental growth
response to glucocorticoid
positive regulation of ERK1 and ERK2 cascade
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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