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Invitrogen
Complement C3 Polyclonal Antibody
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FIGURE: 1 / 4
Complement C3 Antibody (PA5-114921) in ICC/IF
Immunocytochemistry analysis of Complement C3 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114921) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated go... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-114921
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human C3(Accession P01024), corresponding to amino acid residues V944-A994.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total C3.
Target Information
C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; Complement C3; HSE-MSF
Gene Aliases: AI255234; ASP; C3; CPAMD1; HSE-MSF; Plp
UniProt ID: (Rat) P01026, (Mouse) P01027
Entrez Gene ID: (Rat) 24232, (Mouse) 12266
positive regulation of type IIa hypersensitivity
positive regulation of protein phosphorylation
positive regulation of activation of membrane attack complex
tolerance induction
fatty acid metabolic process
chemotaxis
inflammatory response
complement activation
complement activation, alternative pathway
complement activation, classical pathway
blood coagulation
positive regulation of vascular endothelial growth factor production
positive regulation of glucose transport
regulation of triglyceride biosynthetic process
positive regulation of lipid storage
negative regulation of endopeptidase activity
response to magnesium ion
response to estradiol
response to progesterone
response to estrogen
positive regulation of G-protein coupled receptor protein signaling pathway
positive regulation of angiogenesis
positive regulation of developmental growth
positive regulation of phagocytosis
response to glucocorticoid
positive regulation of ERK1 and ERK2 cascade
positive regulation of apoptotic cell clearance
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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