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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunohistochemistry (IHC) |
1:50-1:200 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:200 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1364-1663 of human C3 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.73 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2805376 |
Immunogen sequence: KVTIKPAPET EKRPQDAKNT MILEICTRYR GDQDATMSIL DISMMTGFAP DTDDLKQLAN GVDRYISKYE LDKAFSDRNT LIIYLDKVSH SEDDCLAFKV HQYFNVELIQ PGAVKVYAYY NLEESCTRFY HPEKEDGKLN KLCRDELCRC AEENCFIQKS DDKVTLEERL DKACEPGVDY VYKTRLVKVQ LSNDFDEYIM AIEQTIKSGS DEVQVGQQRT FISPIKCREA LKLEEKKHYL MWGLSSDFWG EKPNLSYIIG KDTWVEHWPE EDECQDEENQ KQCQDLGAFT ESMVVFGCPN; Positive Samples: HepG2, HL-60, HeLa, THP-1, Mouse liver, Rat liver
C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Acylation stimulating protein; acylation-stimulating protein cleavage product; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3-beta-c; C3a anaphylatoxin; C3adesArg; C3bc; Complement C3; Complement C3 alpha chain; Complement C3 beta chain; Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3c alpha' chain fragment 2; Complement C3d fragment; Complement C3dg fragment; Complement C3f fragment; Complement C3g fragment; complement component C3a; complement component C3b; ENCF-1; ENCF-2; epididymis secretory sperm binding protein Li 62p; HSE-MSF; Neutrophil chemotactic factor-1; Neutrophil chemotactic factor-2; prepro-C3
Gene Aliases: AHUS5; AI255234; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p; HSE-MSF; Plp
UniProt ID: (Rat) P01026, (Human) P01024, (Mouse) P01027
Entrez Gene ID: (Rat) 24232, (Human) 718, (Mouse) 12266
Molecular Function:
protease inhibitor
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