Invitrogen

Complement C3 Polyclonal Antibody

Product Image Gallery

Complement C3 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of human liver cancer cells using C3a Polyclonal antibody (Product # PA5-89132) at dilution of 1:100. Blue: DAPI for nuclear staining.

Complement C3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate using C3a Polyclonal antibody (Product # PA5-89132) at a dilution of 1:100 (40x lens).

Complement C3 Antibody in Western Blot (WB) — Western blot analysis of extracts of various cell lines, using C3a Polyclonal antibody (Product # PA5-89132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.

Product Details

Tested Applications

Dilution

Immunofluorescence (IF)

1:50-1:200

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1364-1663 of human C3

Conjugate

Unconjugated

Form

liquid

Concentration

3.06 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen sequence: KVTIKPAPET EKRPQDAKNT MILEICTRYR GDQDATMSIL DISMMTGFAP DTDDLKQLAN GVDRYISKYE LDKAFSDRNT LIIYLDKVSH SEDDCLAFKV HQYFNVELIQ PGAVKVYAYY NLEESCTRFY HPEKEDGKLN KLCRDELCRC AEENCFIQKS DDKVTLEERL DKACEPGVDY VYKTRLVKVQ LSNDFDEYIM AIEQTIKSGS DEVQVGQQRT FISPIKCREA LKLEEKKHYL MWGLSSDFWG EKPNLSYIIG KDTWVEHWPE EDECQDEENQ KQCQDLGAFT ESMVVFGCPN; Positive Samples: HepG2, HL-60, HeLa, THP-1, Mouse liver, Rat liver

Target Information

C3a is an anaphylatoxin generated through activation of the complement C3, and is a potent chemoattractant for phagocytes, and stimulates chemotaxis and other leukocyte functions. C3a binds to eosinophils, basophils, neutrophils, and differentiated U937 cells, that all express receptors forC3a on their cell surface. The C3a receptor has been cloned and shown to be a G protein-coupled receptor with seven putative transmembrane domains. This receptor is characterized for its large extracellular loop between the fourth and fifth transmembrane domains. The loop can be readily detected using the rabbit anti-C3a receptor antibody.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Acylation stimulating protein; acylation-stimulating protein cleavage product; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3-beta-c; C3a anaphylatoxin; C3adesArg; C3bc; Complement C3; Complement C3 alpha chain; Complement C3 beta chain; Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3c alpha' chain fragment 2; Complement C3d fragment; Complement C3dg fragment; Complement C3f fragment; Complement C3g fragment; complement component C3a; complement component C3b; ENCF-1; ENCF-2; epididymis secretory sperm binding protein Li 62p; HSE-MSF; Neutrophil chemotactic factor-1; Neutrophil chemotactic factor-2; prepro-C3

Gene Aliases: AHUS5; AI255234; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p; HSE-MSF; Plp

UniProt ID: (Rat) P01026, (Human) P01024, (Mouse) P01027

Entrez Gene ID: (Rat) 24232, (Human) 718, (Mouse) 12266

Molecular Function: complement component cytokine defense/immunity protein enzyme modulator protease inhibitor serine protease inhibitor signaling molecule