Search
Invitrogen
Complement C3b Monoclonal Antibody (755)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 2
Complement C3b Antibody (MA1-40155) in WB
Western blot was performed using Anti-Complement C3b Monoclonal Antibody (755) (Product # MA1-40155) and a 187 kDa band corresponding to Complement C3 was observed in HepG2 in comparison to HEK-293 which is reported to be negative. A band around 70 kDa was also observed in Hep G2 treated with LPS and PTI which can be the processed form of complement C3. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with LPS (10 ng/mL for 18 hr) followed by protein transport inhibitor (1X for 4 hr) (Lane 2) and HEK-293 (Lane 3) were ... View More
Product Details
MA1-40155
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Fungi
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
755
Immunogen
Native C3
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 0.1% BSA
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-40155 detects C3/C3b from human samples.
MA1-40155 has been successfully used in ELISA and Western blot applications. In western blot, the band size is ~190 kDa under non-reducing and ~100 kDa under reducing conditions.
The MA1-40155 immunogen is native C3.
Positive control: Human serum; Negative control: C3 deficient serum
Target Information
Inactive complement component 3b (iC3b) is a 42kDa neoantigen present in blood serum. Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin. This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated. iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor providing a link between the innate and adaptive immune responses. Additionally iC3b acts as an opsonin which is recognised by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells. iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Complement C3; complement component 3; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; iC3b; prepro-C3; unnamed protein product
Gene Aliases: AHUS5; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p
UniProt ID: (Human) P01024
Entrez Gene ID: (Human) 718
positive regulation of type IIa hypersensitivity
complement activation, lectin pathway
activation of membrane attack complex
positive regulation of protein phosphorylation
positive regulation of activation of membrane attack complex
immune system process
complement receptor mediated signaling pathway
lipid metabolic process
fatty acid metabolic process
inflammatory response
immune response
complement activation
complement activation, alternative pathway
complement activation, classical pathway
signal transduction
G-protein coupled receptor signaling pathway
opsonization
response to bacterium
positive regulation of vascular endothelial growth factor production
positive regulation of glucose transport
regulation of triglyceride biosynthetic process
positive regulation of lipid storage
neuron remodeling
leukocyte chemotaxis
killing of cells of other organism
oviduct epithelium development
B cell activation
innate immune response
positive regulation of G-protein coupled receptor protein signaling pathway
positive regulation of angiogenesis
positive regulation of receptor-mediated endocytosis
positive regulation of phagocytosis
protein maturation
positive regulation of phagocytosis, engulfment
beta-amyloid clearance
complement-dependent cytotoxicity
complement-mediated synapse pruning
vertebrate eye-specific patterning
complement activation, GZMK pathway
positive regulation of apoptotic cell clearance
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support