ELISA: Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:8,000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction. Appropriate specificity controls were run.
C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds.
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Protein Aliases: Acylation stimulating protein; ASP; C3-beta-c; C3a anaphylatoxin; C3adesArg; C3bc; Cd3; Complement C3; Complement C3 alpha chain; Complement C3 beta chain; Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3c alpha' chain fragment 2; Complement C3d fragment; Complement C3dg fragment; Complement C3f fragment; Complement C3g fragment; complement component 3
Gene Aliases: C3
Entrez Gene ID: (Bovine) 280677