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Invitrogen
Complement C4b Polyclonal Antibody
FIGURE: 1 / 1
Complement C4b Antibody (PA1-9534) in WB
Clinical proteomics 2022 -
Fig. 7 Western blot against the mature C4B protein chain in human EDTA plasma over the time course of degradation at room temperature. The monoclonal antibody against C4B was biotinylated and detected with Streptavidin-HRP. The arrow indicates the expected C4B peptide after proteolysis. Lanes: MW, Molecular weight marker as indicated; A, Streptavidin HRP alone; 1, time 0; 2, 1 h; 3, 1 h; 4, 4 h; 5, 8 h; 6, ... View More
Product Details
PA1-9534
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
Purified human C4b complement protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
IgY fraction
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2066765
Product Specific Information
PA1-9534 detects C4b complement in human samples.^M
^M
PA1-9534 has been successfully used in Western blot and ELISA procedures. By Western blot, PA1-9534 detects a 88 Kda, 75 Kda, 33 Kda protein representing C4b complement.^M
^M
PA1-9534 immunogen is a purified human C4b complement protein.^M
^M
Store product at 4ºC for up to one week and -20ºC for long-term.
Target Information
This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Acidic complement C4; Basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3; c4 complement; C4A13; C4A91; Chido form of C4; Complement C4-A; Complement C4-B; complement C4B1a; DADB-112B14.11; FLJ60561; MGC164979
Gene Aliases: C4A; C4B; C4B1; C4B12; C4B2; C4B3; C4B5; C4B_2; C4BD; C4F; CH; CO4; CPAMD2; CPAMD3
UniProt ID: (Human) P01028, (Human) P0C0L5
Entrez Gene ID: (Human) 721, (Human) 100293534
proteolysis
inflammatory response
complement activation
complement activation, classical pathway
opsonization
negative regulation of endopeptidase activity
regulation of complement activation
detection of molecule of bacterial origin
innate immune response
positive regulation of apoptotic cell clearance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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