Immunohistochemical staining of formalin-fixed, paraffin-embedded human kidney (patient with Lupus erythematosus) using a Complement C4d polyclonal antibody (Product # PA1-85168) following Sodium citrate (pH6.0) mediated antigen retrieval. Slide under high power magnificaiton.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 1241-1256 of C4 conjugated to Keyhole limpet Haemocyanin.|
|Contains||0.1% Proclin 300|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:20-1:80|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:80|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval using Tris/EDTA (pH 8.5) is recommended for the staining of paraffin sections.
This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.