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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
C4 is a component of the classical complement pathway and is split by activated C1s to C4a and C4b. Together C2a and C4b forms the classical C3 convertase. The molecular mass of C4 is 204 kDa and it consists of 3 subunits: a (97 kDa), b (75 kDa) and g (33 kDa).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Acidic complement C4; Basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3; c4 complement; C4D; Complement C4-A; Complement C4-B; DADB-112B14.11; FLJ60561; MGC164979
Gene Aliases: C4A; C4B; C4B_2; CO4; CPAMD2; CPAMD3
Entrez Gene ID: (Human) 720, (Human) 721, (Human) 100293534
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