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Invitrogen
Complement C5 Polyclonal Antibody
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FIGURE: 1 / 2
Complement C5 Antibody (PA5-96933) in WB
Western blot analysis of Complement C5 in mouse lung. Samples were incubated with Complement C5 Polyclonal antibody (Product # PA5-96933) using a dilution of 1:600, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-96933
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
0.42 mg/mL
Amount
42 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: U-937, Raji, Jurkat, Mouse heart, Rat liver
Target Information
C5 (Complement C5) is a Protein Coding gene. Diseases associated with C5 include Complement Component 5 Deficiency and Eculizumab, Poor Response To. Among its related pathways are GPCR downstream signalling and Acquired partial lipodystrophy / Barraquer-Simons syndrome. Gene Ontology (GO) annotations related to this gene include signaling receptor binding and endopeptidase inhibitor activity. An important paralog of this gene is C3. This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: anaphylatoxin; anaphylatoxin C5; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; C5a anaphylatoxin; Complement C5; Complement Component 5; CPAMD4; prepro-C5
Gene Aliases: C5; C5a; C5b; C5D; CPAMD4; ECLZB; Hc; RGD1561905
UniProt ID: (Human) P01031
Entrez Gene ID: (Rat) 362119, (Human) 727
in utero embryonic development
leukocyte migration involved in inflammatory response
cellular calcium ion homeostasis
chemotaxis
inflammatory response
complement activation, alternative pathway
complement activation, classical pathway
positive regulation of vascular endothelial growth factor production
negative regulation of norepinephrine secretion
negative regulation of macrophage chemotaxis
negative regulation of dopamine secretion
glucose homeostasis
positive regulation of angiogenesis
positive regulation of chemotaxis
positive regulation of chemokine secretion
complement activation, lectin pathway
immune system process
regulation of immune system process
complement activation
signal transduction
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
cell migration
leukocyte chemotaxis
killing of cells of other organism
positive regulation of chemokine production
innate immune response
regulation of chemotaxis
cell chemotaxis
complement activation, GZMK pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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