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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 22-265 of human C9. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1.29 mg/mL |
Purification |
Affinity Chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2808172 |
Immunogen sequence: QYTTSYDPEL TESSGSASHI DCRMSPWSEW SQCDPCLRQM FRSRSIEVFG QFNGKRCTDA VGDRRQCVPT EPCEDAEDDC GNDFQCSTGR CIKMRLRCNG DNDCGDFSDE DDCESEPRPP CRDRVVEESE LARTAGYGIN ILGMDPLSTP FDNEFYNGLC NRDRDGNTLT YYRRPWNVAS LIYETKGEKN FRTEHYEEQI EAFKSIIQEK TSNFNAAISL KFTPTETNKA EQCCEETASS ISLH; Positive Samples: Mouse liver; Cellular Location: Cell membrane, Multi-pass membrane protein
C5b-9 membrane attack complexes are assembled from five precursor molecules in the serum. Proteolytic cleavage of C5 by C5 convertase generates C5b which initiates assembly of the C5b-9 complex. The last step of C5b-9 complex formation involves polymerization of C9 which accompanies insertion of the complex into the cell membrane. During formation of C5b-8 and C9 polymerization, neoantigens are generated which are unique to the C5b-9 complex and are not present on any of the individual native complex components. The complement regulatory proteins CD59 and complement S-protein can both prevent C5b-9 insertion into the cell membrane. The formed SC5b-9 complex is unable to attach to cells and is cytolytically inactive. C5b-9 is involved in the progression of chronic proteinuric renal disease by mediating continuous tubulointerstitial damage. Early tubulointerstitial injury in the remnant kidney can be improved when C5b-9 complex forming is abrogated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Complement component C9; Complement component C9a; Complement component C9b
Gene Aliases: ARMD15; C9; C9D
UniProt ID: (Human) P02748, (Mouse) P06683
Entrez Gene ID: (Human) 735, (Mouse) 12279
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