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Invitrogen
Complement C9 Monoclonal Antibody (2A1)
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Product Details
MA5-33355
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2A1
Immunogen
Rat C5b-9
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
Protein G
Storage buffer
PBS with 0.1% BSA
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Product is supplied at 1 mL (100 µL/mL). This antibody was shown to compete with antibodies to human C9 for its binding site on the C5b-9 complex, indicating that the reactive epitope is located on the C9 molecule. This product can be used as a coating antibody to detect C5b-9 in plasma and urine samples.
Application Notes: ELISA - plates were coated with 30 µg/mL in 50mM carbonatebuffer, pH10.6 for 16h at 4°C. Western Blot - A non-reduced sample treatment and SDS-Page was used. The band size is >200 kDa. IHC-P - Tissue sections fixed in formalin were pretreated with protease type XXIV for 10 minutes at 37°C before incubation. IHC-F- Tissue sections were fixed in acetone for 10 minutes at room temperature before incubation.
Target Information
C5b-9 membrane attack complexes are assembled from five precursor molecules in the serum. Proteolytic cleavage of C5 by C5 convertase generates C5b which initiates assembly of the C5b-9 complex. The last step of C5b-9 complex formation involves polymerization of C9 which accompanies insertion of the complex into the cell membrane. During formation of C5b-8 and C9 polymerization, neoantigens are generated which are unique to the C5b-9 complex and are not present on any of the individual native complex components. The complement regulatory proteins CD59 and complement S-protein can both prevent C5b-9 insertion into the cell membrane. The formed SC5b-9 complex is unable to attach to cells and is cytolytically inactive. C5b-9 is involved in the progression of chronic proteinuric renal disease by mediating continuous tubulointerstitial damage. Early tubulointerstitial injury in the remnant kidney can be improved when C5b-9 complex forming is abrogated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Complement component C9
Gene Aliases: C9
UniProt ID: (Rat) Q62930
Entrez Gene ID: (Rat) 117512
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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