|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (PFA fixed) (IHC (PFA))||1-3 µg/mL|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-3 µg/mL|
|Western Blot (WB)||See 2 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Mouse Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal region of mouse connexin 31 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody reacts with the ~32 kDa mouse Connexin 31 on Western blots; the band observed at ~62 kDa is probably due to dimerization of Connexin 31. Several higher bands of unknown origin are observed in western blots of mouse skin homogenates.
Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. Defects in GJB3 have been linked to erythrokeratodermia variabilis (EKV) is an autosomal dominant genodermatosis characterized by transient figurate red patches or hyperkeratosis. Mutations in GJB2 have also been associated with genetically derived hearing impairments, including autosomal recessive nonsyndromic deafness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Connexin 31; Connexin-31; Cx31; Gap junction beta-3 protein; gap junction membrane channel protein beta 3; gap junction protein, beta 3, 31kDa
Gene Aliases: Cnx31; CX31; Cxn-31; D4Wsu144e; DFNA2; DFNA2B; EKV; Gjb-3; GJB3
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