Invitrogen

Connexin 36 Polyclonal Antibody

Product Image Gallery

Connexin 36 Antibody — Published figure using Connexin 36 polyclonal antibody (Product # 51-6200)

Connexin 36 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of Connexin 36/GJA9 was done on 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Connexin 36/GJA9 Rabbit polyclonal Antibody (Product # 51-6200) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing junctional localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.

Product Details

Tested Applications

Dilution

ELISA (ELISA)

0.1-1.0 µg/mL

Immunocytochemistry (ICC)

1:100-1:500

Immunofluorescence (IF)

1:100-1:500

Western Blot (WB)

1.0 µg/mL

Published Applications

Immunocytochemistry (ICC)

See 2 publications below

Western Blot (WB)

See 5 publications below

Miscellaneous PubMed (MISC)

See 2 publications below

Immunofluorescence (IF)

See 2 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Non-human primate, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide corresponding to a sequence located in the cytoplasmic loop between the second and third transmembrane domains of rat and mouse Connexin 36 (Cx36). This sequence differs from the human Cx36 protein by a single amino acid.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533912

Target Information

GJA9, also called connexin-36 (CX36), is a member of the connexin gene family that is expressed predominantly in mammalian neurons. Connexins associate in groups of 6 and are organized radially around a central pore to form connexons. Each gap junction intercellular channel is formed by the conjunction of 2 connexons.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: connexin 36; Connexin-36; Cx36; Gap junction alpha-9 protein; Gap junction delta-2 protein; gap junction membrane channel protein alpha 9; gap junction protein, alpha 9; gap junction protein, delta 2, 36kDa; GJA10

Gene Aliases: connexin36; CX36; GJA9; GJD2

UniProt ID: (Human) Q9UKL4, (Rat) O70610, (Mouse) O54851

Entrez Gene ID: (Human) 57369, (Rat) 50564, (Mouse) 14617

Molecular Function: cell junction protein gap junction