Invitrogen

Connexin 36 Polyclonal Antibody

11 Published Figures

19 References

View all (13) Connexin 36 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (11)

Connexin 36 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

Connexin 36 Antibody (51-6200) in ICC/IF

Immunofluorescence analysis of Connexin 36/GJA9 was done on 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Connexin 36/GJA9 Rabbit polyclonal Antibody (Product # 51-6200) at 2 µg/mL in 1% BSA and incubated for 3 hours ... View More

Connexin 36 Antibody in Immunohistochemistry (IHC)

Connexin 36 Antibody in Immunocytochemistry, Immunohistochemistry (PFA fixed) (ICC/IF, IHC (PFA))

Connexin 36 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Connexin 36 Antibody in Western Blot (WB)

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Product Details

51-6200

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

1:100-1:500

View 6 publications 6 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:500

View 4 publications 4 publications

ELISA (ELISA)

0.1-1.0 µg/mL

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide corresponding to a sequence located in the cytoplasmic loop between the second and third transmembrane domains of rat and mouse Connexin 36 (Cx36). This sequence differs from the human Cx36 protein by a single amino acid.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533912

Target Information

Connexin-36 (CX36), is a member of the connexin gene family that is expressed predominantly in mammalian neurons. Connexins associate in groups of 6 and are organized radially around a central pore to form connexons. Each gap junction intercellular channel is formed by the conjunction of 2 connexons. Mutations in connexin-encoding genes can lead to functional and developmental abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An offset ON-OFF receptive field is created by gap junctions between distinct types of retinal ganglion cells.

Nature neuroscience

Cooler S,Schwartz GW

51-6200 was used in Immunohistochemistry to highlight a distinct retinal ganglion cell network forming novel electrical channel in the output layer of the vertebrate retina.

Fri Jan 01 00:00:00 UTC 2021

An offset ON-OFF receptive field is created by gap junctions between distinct types of retinal ganglion cells.

Nature neuroscience

Cooler S,Schwartz GW

51-6200 was used in Immunohistochemistry to highlight a distinct retinal ganglion cell network forming novel electrical channel in the output layer of the vertebrate retina.

Fri Jan 01 00:00:00 UTC 2021

An offset ON-OFF receptive field is created by gap junctions between distinct types of retinal ganglion cells.

Nature neuroscience

Cooler S,Schwartz GW

51-6200 was used in Immunohistochemistry to highlight a distinct retinal ganglion cell network forming novel electrical channel in the output layer of the vertebrate retina.

Fri Jan 01 00:00:00 UTC 2021

Inner retinal preservation in the photoinducible I307N rhodopsin mutant mouse, a model of autosomal dominant retinitis pigmentosa.

The Journal of comparative neurology

Stefanov A,Novelli E,Strettoi E

51-6200 was used in Immunohistochemistry to study inner retinal remodeling in an adult I307N Tvrm4 mouse model of retinitis pigmentosa, at early stages following complete photoreceptor degeneration.

Mon Jun 15 00:00:00 UTC 2020

Brn3a and Brn3b knockout mice display unvaried retinal fine structure despite major morphological and numerical alterations of ganglion cells.

The Journal of comparative neurology

Ghinia MG,Novelli E,Sajgo S,Badea TC,Strettoi E

51-6200 was used in Immunohistochemistry to demonstrate that despite ganglion cell losses, retinal organization in Brn3 null mice is remarkably similar to that of wild-type controls.

Tue Jan 01 00:00:00 UTC 2019

A Self-Regulating Gap Junction Network of Amacrine Cells Controls Nitric Oxide Release in the Retina.

Neuron

Jacoby J,Nath A,Jessen ZF,Schwartz GW

51-6200 was used in Immunohistochemistry to show that a single type of amacrine cell (AC) controls nitric oxide release in the inner retina.

Wed Dec 05 00:00:00 UTC 2018

Differential expression of astrocytic connexins in a mouse model of prenatal alcohol exposure.

Neurobiology of disease

Ramani M,Mylvaganam S,Krawczyk M,Wang L,Zoidl C,Brien J,Reynolds JN,Kapur B,Poulter MO,Zoidl G,Carlen PL

51-6200 was used in western blot to study differential expression of astrocytic connexins by a prenatal alcohol exposure mouse model

Fri Jul 01 00:00:00 UTC 2016

Astrocyte sigma-1 receptors modulate connexin 43 expression leading to the induction of below-level mechanical allodynia in spinal cord injured mice.

Neuropharmacology

Choi SR,Roh DH,Yoon SY,Kwon SG,Choi HS,Han HJ,Beitz AJ,Lee JH

516200 was used in western blot to assess the contributions of Sig-1Rs and connexin 43 to the induction of central neuropathic pain during spinal cord injury

Thu Dec 01 00:00:00 UTC 2016

Glibenclamide Prevents Diabetes in NOD Mice.

PloS one

Lamprianou S,Gysemans C,Bou Saab J,Pontes H,Mathieu C,Meda P

Published figure using Connexin 36 polyclonal antibody (Product # 51-6200) in Immunocytochemistry

Tue Jul 18 00:00:00 UTC 2017

Glibenclamide Prevents Diabetes in NOD Mice.

PloS one

Lamprianou S,Gysemans C,Bou Saab J,Pontes H,Mathieu C,Meda P

Published figure using Connexin 36 polyclonal antibody (Product # 51-6200) in Immunocytochemistry

Tue Jul 18 00:00:00 UTC 2017

Influence of the β2-Subunit of L-Type Voltage-Gated Cav Channels on the Structural and Functional Development of Photoreceptor Ribbon Synapses.

Investigative ophthalmology & visual science

Katiyar R,Weissgerber P,Roth E,Dörr J,Sothilingam V,Garcia Garrido M,Beck SC,Seeliger MW,Beck A,Schmitz F,Flockerzi V

51-6200 was used in Immunocytochemistry to study the altered functions of photoreceptor ribbon synapses lacking the Β2 subunit of voltage-gated Ca2+ channels.

Wed Apr 01 00:00:00 UTC 2015

Suppression of spinal connexin 43 expression attenuates mechanical hypersensitivity in rats after an L5 spinal nerve injury.

Neuroscience letters

Xu Q,Cheong YK,He SQ,Tiwari V,Liu J,Wang Y,Raja SN,Li J,Guan Y,Li W

Published figure using Connexin 36 polyclonal antibody (Product # 51-6200) in Western Blot

Wed Apr 30 00:00:00 UTC 2014

Connexin and AMPA receptor expression changes over time in the rat olfactory bulb.

Neuroscience

Corthell JT,Fadool DA,Trombley PQ

51-6200 was used in western blot to test if circadian electrical oscillations are synchronized by changes in the expression of connexins and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subunits.

Thu Oct 11 00:00:00 UTC 2012

Connexins protect mouse pancreatic β cells against apoptosis.

The Journal of clinical investigation

Klee P,Allagnat F,Pontes H,Cederroth M,Charollais A,Caille D,Britan A,Haefliger JA,Meda P

Published figure using Connexin 36 polyclonal antibody (Product # 51-6200) in Western Blot

Thu Dec 01 00:00:00 UTC 2011

Electrical synapses in retinal ON cone bipolar cells: subtype-specific expression of connexins.

Proceedings of the National Academy of Sciences of the United States of America

Han Y,Massey SC

Tue Sep 13 00:00:00 UTC 2005

Electrical synapses in retinal ON cone bipolar cells: subtype-specific expression of connexins.

Proceedings of the National Academy of Sciences of the United States of America

Han Y,Massey SC

Tue Sep 13 00:00:00 UTC 2005

Morphological impairments in retinal neurons of the scotopic visual pathway in a monkey model of Parkinson's disease.

The Journal of comparative neurology

Cuenca N,Herrero MT,Angulo A,de Juan E,Martínez-Navarrete GC,López S,Barcia C,Martín-Nieto J

51-6200 was used in immunohistochemistry to study amacrine cells in macaque model of Parkinson's disease.

Mon Dec 12 00:00:00 UTC 2005

Ultrastructural localization of connexins (Cx36, Cx43, Cx45), glutamate receptors and aquaporin-4 in rodent olfactory mucosa, olfactory nerve and olfactory bulb.

Journal of neurocytology

Rash JE,Davidson KG,Kamasawa N,Yasumura T,Kamasawa M,Zhang C,Michaels R,Restrepo D,Ottersen OP,Olson CO,Nagy JI

Published figure using Connexin 36 polyclonal antibody (Product # 51-6200) in Immunofluorescence

Thu Sep 01 00:00:00 UTC 2005

Connexin 36 in photoreceptor cells: studies on transgenic rod-less and cone-less mouse retinas.

Molecular vision

Dang L,Pulukuri S,Mears AJ,Swaroop A,Reese BE,Sitaramayya A

51-6200 was used in immunohistochemistry - frozen section to study expression of connexin 36 in transgenic rod-less and cone-less mice

Tue May 11 00:00:00 UTC 2004

Heterogeneous expression of connexin 36 in the olfactory epithelium and glomerular layer of the olfactory bulb.

The Journal of comparative neurology

Zhang C,Restrepo D

Published figure using Connexin 36 polyclonal antibody (Product # 51-6200) in Immunofluorescence

Mon May 12 00:00:00 UTC 2003

Immunogold evidence that neuronal gap junctions in adult rat brain and spinal cord contain connexin-36 but not connexin-32 or connexin-43.

Proceedings of the National Academy of Sciences of the United States of America

Rash JE,Staines WA,Yasumura T,Patel D,Furman CS,Stelmack GL,Nagy JI

Tue Jun 20 00:00:00 UTC 2000

Bioinformatics

Protein Aliases: connexin 36; Connexin-36; Cx36; Gap junction alpha-9 protein; Gap junction delta-2 protein; gap junction membrane channel protein alpha 9; gap junction protein, alpha 9; gap junction protein, delta 2, 36kDa; GJA10

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Gene Aliases: connexin36; CX36; GJA9; GJD2

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UniProt ID: (Human) Q9UKL4, (Rat) Q9ER25, (Mouse) O54851

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Entrez Gene ID: (Human) 57369, (Rat) 50564, (Mouse) 14617

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Function(s)

gap junction channel activity protein binding gap junction

Process(es)

action potential chemical synaptic transmission visual perception transmembrane transport cell-cell signaling cell communication

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Connexin 36 Polyclonal Antibody

Connexin 36 Antibody (51-6200) in ICC/IF

Product Details

51-6200

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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