Immunofluorescence analysis of Connexin 36/GJA9 was done on 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Connexin 36/GJA9 Rabbit polyclonal Antibody (516200) at 2µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing junctional localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Non-human primate, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to a sequence located in the cytoplasmic loop between the second and third transmembrane domains of rat and mouse Connexin 36 (Cx36). This sequence differs from the human Cx36 protein by a single amino acid.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
GJA9, also called connexin-36 (CX36), is a member of the connexin gene family that is expressed predominantly in mammalian neurons. Connexins associate in groups of 6 and are organized radially around a central pore to form connexons. Each gap junction intercellular channel is formed by the conjunction of 2 connexons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Differential expression of astrocytic connexins in a mouse model of prenatal alcohol exposure.
51-6200 was used in western blot to study differential expression of astrocytic connexins by a prenatal alcohol exposure mouse model
|Ramani M,Mylvaganam S,Krawczyk M,Wang L,Zoidl C,Brien J,Reynolds JN,Kapur B,Poulter MO,Zoidl G,Carlen PL||Neurobiology of disease (91:83)||2016|
Suppression of spinal connexin 43 expression attenuates mechanical hypersensitivity in rats after an L5 spinal nerve injury.
51-6200 was used in western blot to show that suppression of spinal connexin 43 after nerve injury alleviates neuropathic mechanical hypersensitivity.
|Xu Q,Cheong YK,He SQ,Tiwari V,Liu J,Wang Y,Raja SN,Li J,Guan Y,Li W||Neuroscience letters (566:194)||2014|
Connexin and AMPA receptor expression changes over time in the rat olfactory bulb.
51-6200 was used in western blot to test if circadian electrical oscillations are synchronized by changes in the expression of connexins and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subunits.
|Corthell JT,Fadool DA,Trombley PQ||Neuroscience (222:38)||2012|
|Rat||Not Cited||Immunogold evidence that neuronal gap junctions in adult rat brain and spinal cord contain connexin-36 but not connexin-32 or connexin-43.||Rash JE,Staines WA,Yasumura T,Patel D,Furman CS,Stelmack GL,Nagy JI||Proceedings of the National Academy of Sciences of the United States of America (97:7573)||2000|
|Non-human primate||Not Cited||
Morphological impairments in retinal neurons of the scotopic visual pathway in a monkey model of Parkinson's disease.
51-6200 was used in immunohistochemistry to study amacrine cells in macaque model of Parkinson's disease.
|Cuenca N,Herrero MT,Angulo A,de Juan E,Martínez-Navarrete GC,López S,Barcia C,Martín-Nieto J||The Journal of comparative neurology (493:261)||2005|
Heterogeneous expression of connexin 36 in the olfactory epithelium and glomerular layer of the olfactory bulb.
51-6200 was used in immunohistochemistry to study the role of connexin 36 in the olfactory epithelium and olfactory bulb of adult mice.
|Zhang C,Restrepo D||The Journal of comparative neurology (459:426)||2003|
|Mouse||1:250||Electrical synapses in retinal ON cone bipolar cells: subtype-specific expression of connexins.||Han Y,Massey SC||Proceedings of the National Academy of Sciences of the United States of America (102:13313)||2005|
|Mouse||Not Cited||Ultrastructural localization of connexins (Cx36, Cx43, Cx45), glutamate receptors and aquaporin-4 in rodent olfactory mucosa, olfactory nerve and olfactory bulb.||Rash JE,Davidson KG,Kamasawa N,Yasumura T,Kamasawa M,Zhang C,Michaels R,Restrepo D,Ottersen OP,Olson CO,Nagy JI||Journal of neurocytology (34:307)||2005|
Connexin 36 in photoreceptor cells: studies on transgenic rod-less and cone-less mouse retinas.
51-6200 was used in immunohistochemistry - frozen section to study expression of connexin 36 in transgenic rod-less and cone-less mice
|Dang L,Pulukuri S,Mears AJ,Swaroop A,Reese BE,Sitaramayya A||Molecular vision (10:323)||2004|