Invitrogen

Connexin 37 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

4 References

View all (7) Connexin 37 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Connexin 37 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Connexin 37 showing staining in the cytoplasm and membrane of paraffin-embedded mouse heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Connexin 37 polyclonal antibody (Product # 42-4400) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Connexin 37 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemical analysis of frozen blood vessels in mouse heart using Zymed Rb anti-Connexin 37 (C-term) (Product # 42-4400). Image courtesy of James I. Nagy, PhD, University of Manitoba, Canada.

Connexin 37 Antibody in Western Blot (WB) — Western blot analysis of Connexin37 (Cx37) was performed by loading 20 µg of protein from mouse lung homogenate and 7 µL of EZ-RUN Prestained Rec Protein Ladder per well onto a 10% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked in blocking buffer composed of 20 mM Tris-HCl pH 8, 150 mM NaCl and 0.2% Tween-20 (TBST) supplemented with 5% skim milk powder (Carnation) for 1 hr at room temperature. Cx37 was detected at ~37 kDa using an anti-Cx37 rabbit polyclonal antibody (Product # 42-4400) at a dilution of 2 µg/mL in TBST supplemented with 1% skim milk powder and incubated overnight at 4C on a rocking platform, followed by washing and incubation with a goat anti-rabbit IgG horse radish peroxidase (HRP) conjugated secondary antibody at a dilution of 1:10,000 in TBST for 1 hr at room temperature. Chemiluminescent detection was performed using Pierce ECL Western blotting substrate (Product # 32209). Data courtesy of Dr. James Nagy's lab.

Connexin 37 Antibody — Protein levels of Cx40, Cx37, Cx43, and Cx45 in MCECs. A and D: typical WB images of Cx40, Cx37, Cx43, and Cx45 protein levels in the whole lysate of MCECs isolated from WT, Cx40 KO, and Cx37 KO mice. Actin was used as a loading control for samples of whole lysate from MCECs. B: Cx40 protein level normalized to actin: WT (n = 5) and Cx40 KO (n = 5). Data are means ± SE. C: Cx37 protein levels in MCECs of WT and Cx40 KO mice (n = 4 in each group). Data are means ± SE. E: Cx43 protein levels in MCECs of WT and Cx40 KO mice (n = 5 in each group). Data are means ± SE. F: Cx45 protein levels in MCECs of WT and Cx40 KO mice (n = 6 in each group). Data are means ± SE. G: photomicrographs show typical image of Cx40 expression in WT MCECs (left) and Cx40 KO MCECs (right). MCECs were stained with Cx40 (green) and Tom20 (a marker of mitochondria; red). {KD}

Product Details

42-4400

Applications	Tested Dilution	Publications
Western Blot (WB)	2 µg/mL	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:10-1:50	-
Immunohistochemistry (Frozen) (IHC (F))	1:10-1:50	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide derived from the C-terminal region of the mouse, rat, golden and Chinese hamster, and predicted dog connexin 37 proteins, which differ from human and chimpanzee by two conservative amino acid changes View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2533524

Product Specific Information

42-4400 was used in the western blot analysis to successfully detect Connexin 37 in mouse lung homogenate.

Target Information

This gene encodes a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene have been associated with atherosclerosis and a higher risk of myocardial infarction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mitochondrial connexin40 regulates mitochondrial calcium uptake in coronary endothelial cells.

American journal of physiology. Cell physiology

Guo R,Si R,Scott BT,Makino A

424400 was used in western blot to determine the role of mitochondrial Cx40 in endothelial cells

Sat Apr 01 00:00:00 EDT 2017

NO, via its target Cx37, modulates calcium signal propagation selectively at myoendothelial gap junctions.

Cell communication and signaling : CCS

Pogoda K,Füller M,Pohl U,Kameritsch P

42-4400 was used in immunocytochemistry to study the effect of NO on the gap junctional calcium signal propagation in endothelial cells and determine the distribution of the vascular connexins in small resistance arteries.

Thu May 15 00:00:00 EDT 2014

TIMP-2 mutant decreases MMP-2 activity and augments pressure overload induced LV dysfunction and heart failure.

Archives of physiology and biochemistry

Givvimani S,Kundu S,Narayanan N,Armaghan F,Qipshidze N,Pushpakumar S,Vacek TP,Tyagi SC

Wed May 01 00:00:00 EDT 2013

Cx37 and Cx43 localize to zona pellucida in mouse ovarian follicles.

Cell communication & adhesion

Simon AM,Chen H,Jackson CL

Thu Aug 10 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: connexin 37; Connexin-37; Cx37; Gap junction alpha-4 protein; gap junction membrane channel protein alpha 4; gap junction protein, alpha 4, 37kDa

Gene Aliases: AU020209; AW558810; Cnx37; CX37; Cxn-37; Gja-4; GJA4

UniProt ID: (Human) P35212, (Mouse) P28235

Entrez Gene ID: (Human) 2701, (Mouse) 14612

Molecular Function: gap junction