Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunohistochemistry analysis of Connexin 47 showing staining in the membrane and cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Connexin 47 polyclonal antibody (364700) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human , Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal region of the mouse Connexin 47 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||1-2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
36-4700 was used in immunofluorescence analysis of Connexin47 (Cx47) on sections of adult mouse brain.
This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Absence of venous valves in mice lacking Connexin37.
36-4700 was used in immunohistochemistry to investigate the role of Cx37 during vasculogenesis in mice.
|Munger SJ,Kanady JD,Simon AM||Developmental biology (373:338)||2013|
Pathologic and phenotypic alterations in a mouse expressing a connexin47 missense mutation that causes Pelizaeus-Merzbacher-like disease in humans.
36-4700 was used in immunohistochemistry - frozen section to characterize Cx47M282T mice.
|Tress O,Maglione M,Zlomuzica A,May D,Dicke N,Degen J,Dere E,Kettenmann H,Hartmann D,Willecke K||PLoS genetics (7:null)||2011|
Ablation of Cx47 in transgenic mice leads to the loss of MUPP1, ZONAB and multiple connexins at oligodendrocyte-astrocyte gap junctions.
||Li X,Penes M,Odermatt B,Willecke K,Nagy JI||The European journal of neuroscience (28:1503)||2008|
Expression of zonula occludens-1 (ZO-1) and the transcription factor ZO-1-associated nucleic acid-binding protein (ZONAB)-MsY3 in glial cells and colocalization at oligodendrocyte and astrocyte gap junctions in mouse brain.
||Penes MC,Li X,Nagy JI||The European journal of neuroscience (22:404)||2005|
GJA12, PMLDAR, CX46.6, LMPH1C, SPG44, Gja12, B230382L12Rik, Cx47, HLD2
connexin 47 type A, connexin 47 type C, connexin 47 type D, connexin-47, gap junction alpha-12 protein, gap junction gamma-2 protein, gap junction membrane channel protein alpha 12, gap junction protein, chi 2, connexin-46.6, Gap junction gamma-2 protein, Cx46.6, Cx47, GJC2, GJA12