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Western blot analysis of Cre recombinase using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of Cre-ERT2-transfected 293T cells (Cre-ERT2 fusion protein size: 72kDa). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Cre recombinase polyclonal antibody (Product # PA5-32245) at a dilution of 1:10000.
|Tested species reactivity||Virus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 133 and 343 of Human Cre|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-32245 targets Cre in WB applications and shows reactivity with Human samples.
The PA5-32245 immunogen is recombinant protein fragment corresponding to a region within amino acids 133 and 343 of Human Cre.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.