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Rockland
Cripto-1 TDGF1 Polyclonal Antibody
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FIGURE: 1 / 1
Cripto-1 TDGF1 Antibody (600-401-997) in WB
Western blot using Rocklands affinity purified anti-Cripto-1 antibody shows detection of endogenous and transfected Cripto-1 from mouse and human sources. The Cripto-1 band appears above the 17.8 kDa marker. Endogenous detection is shown using mouse P19 embryonal carcinoma cells and human NCCIT testicular embryonal carcinoma cells. EpH4 CR-1 is a mouse mammary epithelial cell line stably expressing mouse Cripto-1. NTERA cells are human embryonal carcinoma cells that, when overgrown, differentiate and lose Cripto-1 expression. COS7 cells tr... View More
Product Details
600-401-997
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human Cripto-1 protein.
Conjugate
Form
Liquid
Concentration
0.97 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for human Cripto-1 protein. A BLAST analysis was used to suggest limited cross-reactivity with Cripto-1 from mouse based on a 70% homology with the immunizing sequence. Expect cross-reactivity with human Cripto-3 (TDGF2) based on very high levels of sequence conservation. Cross-reactivity with Cripto-1 from other sources has not been determined.
Target Information
Cripto (TDGF1) is a GPI-anchored cell membrane proetin involved in Nodal signaling. Cell associated TDGF1 acts as a a Nodeal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulated Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. TDGF1 is expressed both in ES cells and during the early phases of embryo development, while in adults it is reactivated in a wide range of epithelial cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CRGF; Cripto, EGF-CFC family member; Cripto-1; Cripto-1 growth factor; Cripto-3; Epidermal growth factor-like cripto protein CR1; Protein Cripto; TDGF2; Teratocarcinoma-derived growth factor 1; unnamed protein product
Gene Aliases: CR; CR-1; CRGF; CRIPTO; CRIPTO-1; TDGF1
UniProt ID: (Human) P13385
Entrez Gene ID: (Human) 6997
blood vessel development
morphogenesis of a branching structure
cell migration involved in sprouting angiogenesis
signal transduction
epidermal growth factor receptor signaling pathway
determination of left/right symmetry
heart development
positive regulation of cell proliferation
anterior/posterior axis specification, embryo
embryo development ending in birth or egg hatching
anterior/posterior pattern specification
regulation of signal transduction
positive regulation of endothelial cell migration
cell differentiation
positive regulation of cell migration
mammary gland development
cellular response to hepatocyte growth factor stimulus
nodal signaling pathway
negative regulation of apoptotic process
positive regulation of MAP kinase activity
positive regulation of MAPK cascade
cellular response to fibroblast growth factor stimulus
cellular response to interferon-gamma
cellular response to interleukin-6
cellular response to tumor necrosis factor
cellular response to epidermal growth factor stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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