Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
PA5-22700
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide made to an N-terminal portion of the murine cripto protein sequence (between residues 1-50).
Conjugate
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
0.94 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
50mM sodium borate
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11152986
Product Specific Information
In Western blot a band is seen at ~18 kDa representing the core form of Cripto1, and at ~36 kDa reporesenting the glycosylated/ post-translationally modified form.
Suggested positive control: MDA-MB231 (human breast cancer) lysates.
Target Information
Cripto (TDGF1) is a GPI-anchored cell membrane proetin involved in Nodal signaling. Cell associated TDGF1 acts as a a Nodeal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulated Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. TDGF1 is expressed both in ES cells and during the early phases of embryo development, while in adults it is reactivated in a wide range of epithelial cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CRGF; Cripto growth factor; Cripto, EGF-CFC family member; Cripto-1; Cripto-1 growth factor; Cripto-3; Epidermal growth factor-like Cripto protein; Epidermal growth factor-like cripto protein CR1; Protein Cripto; TDGF2; Teratocarcinoma-derived growth factor; Teratocarcinoma-derived growth factor 1; TERATOCARCINOMA-DERIVED GROWTH FACTOR PRECURSOR (EPIDERMAL GROWTH FACTOR-LIKE CRIPTO PROTEIN) (CRIPTO GROWTH FACTOR)
Gene Aliases: CR; CR1; CRGF; CRIPTO; CRIPTO-1; TDGF1
UniProt ID: (Human) P13385, (Mouse) P51865
Entrez Gene ID: (Human) 6997, (Mouse) 21667
activation of MAPK activity
morphogenesis of a branching structure
cell migration involved in sprouting angiogenesis
epidermal growth factor receptor signaling pathway
heart development
positive regulation of cell proliferation
anterior/posterior axis specification, embryo
embryo development
regulation of signal transduction
positive regulation of endothelial cell migration
peptidyl-serine phosphorylation
cell differentiation
positive regulation of cell migration
mammary gland development
somatic stem cell population maintenance
cellular response to hepatocyte growth factor stimulus
negative regulation of apoptotic process
cellular response to fibroblast growth factor stimulus
positive regulation of peptidyl-tyrosine phosphorylation
cellular response to interferon-gamma
cellular response to interleukin-6
cellular response to tumor necrosis factor
cellular response to epidermal growth factor stimulus
vasculogenesis
in utero embryonic development
positive regulation of cell-matrix adhesion
gastrulation
anterior/posterior axis specification
regulation of cell migration
BMP signaling pathway
negative regulation of transforming growth factor beta receptor signaling pathway
nodal signaling pathway
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
cardiac muscle cell differentiation
canonical Wnt signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support