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Invitrogen
CtBP2 Polyclonal Antibody
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FIGURE: 1 / 10
CtBP2 Antibody (PA5-79085) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of CtBP2 in MCF-7 cells using CtBP2 Polyclonal Antibody (Product # PA5-79085). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL and mouse anti-Tubulin beta antibody overnight at 4°C. Cy3 conjugated goat anti-rabbit IgG and DyLight 488 conjugated goat anti-mouse IgG were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Vis... View More
Product Details
PA5-79085
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CTBP2 (429-445aa PNQPTKHGDNREHPNEQ).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A549 whole cell, human HEK293 whole cell, human CACO-2 whole cell, human U20S whole cell, rat brain tissue, mouse lung tissue. IHC: human mammary cancer tissue, rat intestine tissue, rat brain tissue, mouse intestine tissue IHC-F: rat intestine tissue, mouse intestine tissue. ICC/IF: MCF-7 cell, human Hela cell. Flow: HEL cell.
Target Information
This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21. 3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-terminal-binding protein 2; CtBP2; gene trap ROSA b-geo 6; ribeye; RP11-59C5.2
Gene Aliases: AA407280; CTBP2; D7Ertd45e; Gtrgeo6; Ribeye
UniProt ID: (Human) A8K2X5, (Rat) Q9EQH5, (Mouse) P56546
Entrez Gene ID: (Human) 1488, (Rat) 81717, (Mouse) 13017
chromatin binding
transcription coactivator activity
transcription corepressor activity
protein binding
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
protein homodimerization activity
retinoic acid receptor binding
NAD binding
oxidoreductase activity
transcription cofactor
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
negative regulation of cell proliferation
viral genome replication
positive regulation of chromatin binding
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of retinoic acid receptor signaling pathway
white fat cell differentiation
oxidation-reduction process
regulation of transcription, DNA-templated
metabolic process
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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