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Bethyl Laboratories
CtIP Polyclonal Antibody
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FIGURE: 1 / 1
CtIP Antibody (A300-487A) in IP
Detection of human CtIP by immunoprecipitation. Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from MCF-7 cells. Antibodies: Affinity purified rabbit anti-CtIP antibody BL1913 (Product # A300-487A) used at 3 µg/mg lysate for IP. CtIP was also immunoprecipitated with rabbit anti-CtIP antibody BL1914 (Product #A300-488A) using 3 µg/mg lysate. Immunoprecipitated CtIP was blotted using BL1914 at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A300-487A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residues 525 and 575 of human CtBP Interacting Protein (Retinoblastoma Binding Protein 8).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
CtIP, a 125 kD protein, was originally found interacting with a transcription repressor, CtBP, through the PLDLS motif (CtBP-Interacting Protein) thus suggested a role in transcription. Studies have shown that CtIP also interacts with BRCA1 protein through the c-terminus BRCT domains also suggested that CtIP is a potential tumor suppressor. This CtIP-BRCA1 interaction can be disrupted by DNA damaging agents including UV or gamma-irradiation. Li et al (Nature 406, 210 - 215 (2000)) have shown that ATM phosphorylates CtIP at serine residues 664 and 745, and mutation of these sites abrogates the dissociation of BRCA1 from CtIP, resulting in persistent repression of BRCA1-dependent induction of GADD45 upon ionizing radiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTBP-interacting protein; CtIP; DNA endonuclease RBBP8; RBBP-8; retinoblastoma binding protein 8; Retinoblastoma-binding protein 8; Retinoblastoma-interacting protein and myosin-like; RIM; SAE2; Sporulation in the absence of SPO11 protein 2 homolog
Gene Aliases: COM1; CTIP; JWDS; RBBP8; RIM; SAE2; SCKL2
UniProt ID: (Human) Q99708
Entrez Gene ID: (Human) 5932
cell cycle checkpoint
G1/S transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
double-strand break repair via homologous recombination
DNA double-strand break processing
DNA synthesis involved in DNA repair
strand displacement
blastocyst hatching
DNA replication
DNA repair
nucleotide-excision repair
regulation of transcription from RNA polymerase II promoter
mitotic nuclear division
DNA double-strand break processing involved in repair via single-strand annealing
G2 DNA damage checkpoint
response to estradiol
cell division
meiotic cell cycle
nucleic acid phosphodiester bond hydrolysis
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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