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          • Primary Antibodies ›
          • Cullin 1 Antibodies

          Bethyl Laboratories

          Cul1 Polyclonal Antibody

          View all (15) Cullin 1 antibodies

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          Cite Cul1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          Cul1 Antibody in Western Blot (WB)
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          Cul1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Cul1 Antibody (A303-373A) in WB

          Detection of human and mouse Cul1 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Cul1 antibody (Product # A303-373A) used for WB at 0.04 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 6 µg/mg lysate. Cul1 was also immunoprecipitated by rabbit anti-Cul1 antibody A303-372A, which recognizes an upstream epitope. Detection: Chemilum... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Cul1 Antibody in Western Blot (WB)
          Cul1 Polyclonal Antibody

          Product Details

          A303-373A

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:10,000
          -

          Immunoprecipitation (IP)

          2-10 µg/mg lysate
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Region between residue 726 and 776 of human Cullin 1.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          tris citrate/phosphate, pH 7-8

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          The recommended shelf life for this product is 1 year from date of receipt.

          Based on 100% sequence identity, this antibody is predicted to react with Orangutan

          Target Information

          CUL1 gene ontology annotations related to this gene include animal organ morphogenesis; apoptotic process; cell proliferation; protein monoubiquitination; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: AT4G02570; ATCUL1; AXR6; CUL-1; Cullin-1; ETA1; ICU13; unnamed protein product

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          Gene Aliases: CUL1

          View more View less

          UniProt ID: (Human) Q13616, (Mouse) Q9WTX6

          View more View less

          Entrez Gene ID: (Human) 8454, (Mouse) 26965

          View more View less

          Function(s)
          protein binding protein binding, bridging ubiquitin protein ligase binding ubiquitin ligase complex scaffold activity ubiquitin protein ligase activity ubiquitin-protein ligase
          Process(es)
          autophagosome assembly G1/S transition of mitotic cell cycle ubiquitin-dependent protein catabolic process protein monoubiquitination apoptotic process lysosome organization cell proliferation organ morphogenesis negative regulation of autophagy positive regulation of autophagy protein ubiquitination SCF-dependent proteasomal ubiquitin-dependent protein catabolic process cellular response to nutrient levels cellular response to oxidative stress TORC1 signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process protein K48-linked ubiquitination intrinsic apoptotic signaling pathway antiviral innate immune response positive regulation of epithelial cell apoptotic process negative regulation of TORC1 signaling viral process protein ubiquitination involved in ubiquitin-dependent protein catabolic process
          It has to be done as per old AB suggested Products section.

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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