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Product Details
A302-475A
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
Assay-dependent | - |
Immunohistochemistry (IHC) |
1:1,000-1:5,000 | - |
Immunoprecipitation (IP) |
2-5 µg/mg lysate | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Between 150 and 200 |
Conjugate |
Unconjugated |
Form |
liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
tris citrate/phosphate, pH 7-8 |
Contains |
0.09% sodium azide |
Storage conditions |
4° C |
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Mouse and Orangutan
Target Information
Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CUL-2; Cullin-2; MGC131970; OTTHUMP00000019437; testis secretory sperm-binding protein Li 238E
Gene Aliases: CUL2
UniProt ID: (Human) Q13617
Entrez Gene ID: (Human) 8453
Molecular Function:
ubiquitin-protein ligase
G1/S transition of mitotic cell cycle
cell cycle arrest
negative regulation of cell proliferation
viral process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
regulation of transcription from RNA polymerase II promoter in response to hypoxia
intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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